Detailed information of TA system TA222114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2784708..2785279
• Replicon: chromosome
• Accession: NZ_CP085841
• Organism: Enterococcus faecalis strain FA2-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LJ570_RS13590
• Protein ID: WP_002385138.1
• Coordinates: 2784708..2785049 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: LJ570_RS13595
• Protein ID: WP_002354773.1
• Coordinates: 2785049..2785279 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJ570_RS13585 (2780723)	2780723..2784337	-	3615	WP_010774058.1	DNA-directed RNA polymerase subunit beta	-
LJ570_RS13590 (2784708)	2784708..2785049	-	342	WP_002385138.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LJ570_RS13595 (2785049)	2785049..2785279	-	231	WP_002354773.1	hypothetical protein	Antitoxin
LJ570_RS13600 (2785685)	2785685..2785900	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
LJ570_RS13605 (2786039)	2786039..2787031	+	993	WP_002385136.1	biotin--[acetyl-CoA-carboxylase] ligase	-
LJ570_RS13610 (2787295)	2787295..2787933	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
LJ570_RS13615 (2788621)	2788621..2790237	+	1617	WP_002372620.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13128.35 Da
• Isoelectric Point: 9.3984

>T222114 WP_002385138.1 NZ_CP085841:c2785049-2784708 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAAICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T222114 NZ_CP117696:330818-331186 [Salmonella enterica subsp. enterica serovar London]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGCGCCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT222114 WP_002354773.1 NZ_CP085841:c2785279-2785049 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT222114 NZ_CP117696:330600-330821 [Salmonella enterica subsp. enterica serovar London]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACACGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAATTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812