Detailed information of TA system TA222084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 662328..662914
• Replicon: chromosome
• Accession: NZ_CP085824
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Ivysaur

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A3V9X0E4
• Locus tag: LJF36_RS03095
• Protein ID: WP_000174964.1
• Coordinates: 662546..662914 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: V7IU48
• Locus tag: LJF36_RS03090
• Protein ID: WP_001522145.1
• Coordinates: 662328..662549 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF36_RS03065 (657349)	657349..658458	+	1110	WP_000822978.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LJF36_RS03070 (658518)	658518..659444	+	927	WP_000003007.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LJF36_RS03075 (659441)	659441..660718	+	1278	WP_000803764.1	branched chain amino acid ABC transporter permease LivM	-
LJF36_RS03080 (660715)	660715..661482	+	768	WP_000082080.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LJF36_RS03085 (661484)	661484..662197	+	714	WP_000416114.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LJF36_RS03090 (662328)	662328..662549	+	222	WP_001522145.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LJF36_RS03095 (662546)	662546..662914	+	369	WP_000174964.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LJF36_RS03100 (663173)	663173..664489	+	1317	WP_000624755.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LJF36_RS03105 (664594)	664594..665481	+	888	WP_000099303.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LJF36_RS03110 (665478)	665478..666323	+	846	WP_000572196.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LJF36_RS03115 (666325)	666325..667395	+	1071	WP_000907840.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	659441..668132	8691

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-114)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13601.91 Da
• Isoelectric Point: 6.7252

>T222084 WP_000174964.1 NZ_CP085824:662546-662914 [Salmonella enterica subsp. enterica serovar Enteritidis]
MTLQLISAEEIIQFHDRLLRVTPGVTGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLAANPDFVDMTVDAAAGRLTLEQIVVRLRA

Nucleotide

• Length: 369 bp

>T222084 NZ_CP117674:17925-18176 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8166.17 Da
• Isoelectric Point: 5.0775

>AT222084 WP_001522145.1 NZ_CP085824:662328-662549 [Salmonella enterica subsp. enterica serovar Enteritidis]
MRTVNYSEARQNLAEVLESAVTAGPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGNELRELADK

Nucleotide

• Length: 222 bp

>AT222084 NZ_CP117674:18163-18390 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Structures

Toxin

• AlphaFold DB: A0A3V9X0E4