Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 345346..345922 Replicon chromosome
Accession NZ_CP085821
Organism Salmonella enterica subsp. enterica serovar Enteritidis strain Charizard

Toxin (Protein)


Gene name shpA Uniprot ID M7RG88
Locus tag LJF63_RS01640 Protein ID WP_001131963.1
Coordinates 345346..345633 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID B5R9R5
Locus tag LJF63_RS01645 Protein ID WP_000063142.1
Coordinates 345620..345922 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF63_RS01620 (342204) 342204..343092 - 889 Protein_314 restriction endonuclease subunit S -
LJF63_RS01625 (343106) 343106..343510 - 405 WP_227957819.1 hypothetical protein -
LJF63_RS01630 (343500) 343500..343679 - 180 Protein_316 DNA methyltransferase -
LJF63_RS01635 (343673) 343673..344929 - 1257 Protein_317 NERD domain-containing protein/DEAD/DEAH box helicase -
LJF63_RS01640 (345346) 345346..345633 + 288 WP_001131963.1 BrnT family toxin Toxin
LJF63_RS01645 (345620) 345620..345922 + 303 WP_000063142.1 BrnA antitoxin family protein Antitoxin
LJF63_RS01650 (345997) 345997..346953 - 957 WP_000187843.1 GTPase -
LJF63_RS01655 (346964) 346964..347167 - 204 WP_000460616.1 YbdD/YjiX family protein -
LJF63_RS01660 (347262) 347262..349412 - 2151 WP_000379928.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11294.80 Da        Isoelectric Point: 9.5894

>T222006 WP_001131963.1 NZ_CP085821:345346-345633 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T222006 NZ_CP117654:2353553-2353831 [Escherichia albertii]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGTTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
CCCCATTAAGCGGCGATCGCGATGGCATCTGGGCAATATCTGTATCAGGCAACTGGCGAATCACGTTTGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11373.96 Da        Isoelectric Point: 10.1293

>AT222006 WP_000063142.1 NZ_CP085821:345620-345922 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp

>AT222006 NZ_CP117654:2353831-2354115 [Escherichia albertii]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGACTGTTGACCGGAAAAGCGGCTTTGACGCCAGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCTTGGAGTCTGGCAGAGGCTGAG
AAAACGGTGGATGTGTCGCGGTTGCGCCGTCTGGTGACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VD7

Antitoxin

Source ID Structure
PDB 7VD7
AlphaFold DB A0A4D6PB01

References