Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 301090..301640 Replicon chromosome
Accession NZ_CP085821
Organism Salmonella enterica subsp. enterica serovar Enteritidis strain Charizard

Toxin (Protein)


Gene name ccdB Uniprot ID M7RJ32
Locus tag LJF63_RS01415 Protein ID WP_001199743.1
Coordinates 301332..301640 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID M7RP97
Locus tag LJF63_RS01410 Protein ID WP_001118105.1
Coordinates 301090..301329 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF63_RS01375 (296529) 296529..297560 - 1032 WP_000453347.1 L-idonate 5-dehydrogenase -
LJF63_RS01380 (297777) 297777..298307 + 531 WP_000896758.1 gluconokinase -
LJF63_RS01385 (298335) 298335..299354 - 1020 WP_000152558.1 NAD(P)-dependent alcohol dehydrogenase -
LJF63_RS01400 (300111) 300111..300542 + 432 Protein_269 helix-turn-helix domain-containing protein -
LJF63_RS23000 (300573) 300573..300656 + 84 WP_228958541.1 DUF4942 domain-containing protein -
LJF63_RS01405 (300733) 300733..300981 + 249 WP_000168388.1 ribbon-helix-helix domain-containing protein -
LJF63_RS01410 (301090) 301090..301329 + 240 WP_001118105.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
LJF63_RS01415 (301332) 301332..301640 + 309 WP_001199743.1 CcdB family protein Toxin
LJF63_RS01420 (302046) 302046..302186 + 141 Protein_274 Arm DNA-binding domain-containing protein -
LJF63_RS01425 (302218) 302218..303351 - 1134 Protein_275 IS3 family transposase -
LJF63_RS01430 (303675) 303675..304205 + 531 WP_000909461.1 SEF14 fimbria major subunit SefA -
LJF63_RS01435 (304391) 304391..305068 + 678 WP_001691890.1 SEF14/SEF18 fimbria chaperone SefB -
LJF63_RS23050 (305085) 305085..305966 + 882 WP_227792933.1 fimbria/pilus outer membrane usher protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 300156..303259 3103


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(9-79)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11845.67 Da        Isoelectric Point: 6.7228

>T222005 WP_001199743.1 NZ_CP085821:301332-301640 [Salmonella enterica subsp. enterica serovar Enteritidis]
MQYMVYRNKGNSKAYPYLLDVQSDIIDELHTRMVIPLFPVSRLVNNPVKRLTPTLNVEGNDYLVMTHEMASIRLSQIGDE
VMDVRSHRQTIKNALDFIFDGF

Download         Length: 309 bp

>T222005 NZ_CP117654:2048696-2048798 [Escherichia albertii]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAAAACA
GGAATCGTTTTCGGTCTCTTTTT

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8719.90 Da        Isoelectric Point: 6.4633

>AT222005 WP_001118105.1 NZ_CP085821:301090-301329 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPGMSVRHKKTVSVTLEPALLQQARDAGINLSAVLTAALKEEISATGVERWKAENRAGLQELNRITDEHGLLSDDYRTF

Download         Length: 240 bp

>AT222005 NZ_CP117654:c2048800-2048568 [Escherichia albertii]
ATAAAAAGAGACCGAAAACGATTCCTGTTTTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTACAAAATGGTCAG
TAAAAAGCGCGTCGGCCATCGTCTTAAATGTTAAAAACCGCCCGTTCTGGTGAAAGAACTGTGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C6Z9V9

Antitoxin

Source ID Structure
AlphaFold DB A0A5H9SZK8

References