Detailed information of TA system TA221956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 541246..541866
• Replicon: chromosome
• Accession: NZ_CP085819
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain Blastoise

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: LJF65_RS02615
• Protein ID: WP_001280991.1
• Coordinates: 541648..541866 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: V1H4V6
• Locus tag: LJF65_RS02610
• Protein ID: WP_000344807.1
• Coordinates: 541246..541620 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF65_RS02600 (536385)	536385..537578	+	1194	WP_001039202.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
LJF65_RS02605 (537601)	537601..540750	+	3150	WP_001132508.1	efflux RND transporter permease AcrB	-
LJF65_RS02610 (541246)	541246..541620	+	375	WP_000344807.1	Hha toxicity modulator TomB	Antitoxin
LJF65_RS02615 (541648)	541648..541866	+	219	WP_001280991.1	HHA domain-containing protein	Toxin
LJF65_RS02620 (542045)	542045..542596	+	552	WP_001278792.1	maltose O-acetyltransferase	-
LJF65_RS02625 (542713)	542713..543183	+	471	WP_000136181.1	YlaC family protein	-
LJF65_RS02630 (543239)	543239..543379	-	141	WP_001197749.1	type B 50S ribosomal protein L36	-
LJF65_RS02635 (543385)	543385..543645	-	261	WP_000801415.1	type B 50S ribosomal protein L31	-
LJF65_RS02640 (543870)	543870..545420	+	1551	WP_000213139.1	EAL domain-containing protein	-
LJF65_RS02650 (545651)	545651..546040	+	390	WP_000961285.1	MGMT family protein	-
LJF65_RS02655 (546073)	546073..546642	-	570	WP_000779803.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T221956 WP_001280991.1 NZ_CP085819:541648-541866 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T221956 NZ_CP117643:2442077-2442463 [Escherichia albertii]
ATGATTTGGGTCAGTGCACAGGAGGTCATTGCCTTCCACGATCGAATTTTGCAACGTTTTCCCGGTGTGGCGGGCCTGAC
TGACCCCGGCAGAGCACAGGCGTTGATTTATCGCGTGCAGAATCGTGTTCACTACGAGGGCGTTACTGACCTGTTTGAAC
TGGCAGCAACCTACTGGGTTGCCATCGCTCGCGGTCACATCTTTCATGATGGTAATAAACGTACCGCATTTTTCGTTACT
ATGACCTTTCTTTGGCGCAATGGCATCCGTATCCGTGACGTTGATAACTCGCTGGAGAACCTGACTGTCGAAGCGGCTAC
CGGAGAAAAAACGGTCGGTCAATTAGCGCGATGTTTACGTGAACGAGTGGATAGTTCCGGGAATTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14453.27 Da
• Isoelectric Point: 5.1444

>AT221956 WP_000344807.1 NZ_CP085819:541246-541620 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT221956 NZ_CP117643:2441859-2442080 [Escherichia albertii]
ATGCAAACTTACACTTCCACACAAGCCAGAGCCAATATTGCCACTGTACTGGATACCGCGTTGAATGGTGAGCCAGTTGA
AATCACCCGTCGCGACGGCAGCTCTGCCGTGCTCATTAGTAAAGCCGCATTCGAGGCGTATCAAAATGCGAAACTGGACG
CGGAATTTGATGCCATCATGCAACGTCATGAACACACAATTGAAGCGTTGACTGATCGATGA

Structures

Toxin

• PDB: 4ICG

Antitoxin

• AlphaFold DB: A0A5I5FGI0