Detailed information of TA system TA221935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 547387..547963
• Replicon: chromosome
• Accession: NZ_CP085818
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Clefairy

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: LJF66_RS02580
• Protein ID: WP_001131963.1
• Coordinates: 547387..547674 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: LJF66_RS02585
• Protein ID: WP_000063142.1
• Coordinates: 547661..547963 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF66_RS02565 (544247)	544247..545551	-	1305	WP_000863534.1	type I restriction-modification protein specificity subunit	-
LJF66_RS02570 (545541)	545541..545720	-	180	Protein_488	DNA methyltransferase	-
LJF66_RS02575 (545714)	545714..546970	-	1257	Protein_489	NERD domain-containing protein/DEAD/DEAH box helicase	-
LJF66_RS02580 (547387)	547387..547674	+	288	WP_001131963.1	BrnT family toxin	Toxin
LJF66_RS02585 (547661)	547661..547963	+	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
LJF66_RS02590 (548038)	548038..548994	-	957	WP_000187843.1	GTPase	-
LJF66_RS02595 (549005)	549005..549208	-	204	WP_000460616.1	YbdD/YjiX family protein	-
LJF66_RS02600 (549303)	549303..551453	-	2151	WP_000379928.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T221935 WP_001131963.1 NZ_CP085818:547387-547674 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T221935 NZ_CP117637:642740-643054 [Escherichia albertii]
ATGCACCTGATCACTCAAAAAGCATTGAAAGATGCAGCGGAAAAATATCCGCAGCATAAAACGGAGTTGGTGGCACTGGG
GAACACGATTGCTAAGGGATACTTCAAAAAACCTGAGTCATTAAAAGCAGTGTTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCACAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT221935 WP_000063142.1 NZ_CP085818:547661-547963 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT221935 NZ_CP117637:643051-643467 [Escherichia albertii]
ATGATTGCGATTGCCGATATCTTGCAAGCAGGAGAAAAGCTAACCGATGTGGCACCTTTTCTGGCGGGTATCCAGAGCGA
AGAACAGTATGCCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAATCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCTGAATTTAATGCGATGGTTCAAGCCATGCCTGGCGGT
GTTGCTGTGATTCGTACCCTGATGGATCAATACGGCTTAACCCTTTCCGATCTTCCAGAAATTGGCAGTAAATCCATGGT
GTCACGCGTTTTGAACGGGAAGAGAAAATTAACGCTGGAACACGCTAAAAAACTGGCAGCGCGATTCGGCATTTCCCCCG
CGTTGTTTATTGATTAA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01