Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4448181..4448941 Replicon chromosome
Accession NZ_CP085816
Organism Salmonella enterica subsp. enterica serovar Enteritidis strain Slowpoke

Toxin (Protein)


Gene name tacT Uniprot ID A0A1R2W0C7
Locus tag LJF68_RS21725 Protein ID WP_000533912.1
Coordinates 4448456..4448941 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID M7RHS4
Locus tag LJF68_RS21720 Protein ID WP_000965886.1
Coordinates 4448181..4448468 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF68_RS21700 (4443586) 4443586..4444497 + 912 WP_001168551.1 glycine--tRNA ligase subunit alpha -
LJF68_RS21705 (4444507) 4444507..4446576 + 2070 WP_001291741.1 glycine--tRNA ligase subunit beta -
LJF68_RS21710 (4446966) 4446966..4447364 + 399 Protein_4221 IS3 family transposase -
LJF68_RS21715 (4447536) 4447536..4448003 + 468 WP_000702452.1 GNAT family N-acetyltransferase -
LJF68_RS21720 (4448181) 4448181..4448468 + 288 WP_000965886.1 DUF1778 domain-containing protein Antitoxin
LJF68_RS21725 (4448456) 4448456..4448941 + 486 WP_000533912.1 GNAT family N-acetyltransferase Toxin
LJF68_RS21730 (4449312) 4449312..4449851 - 540 WP_000047147.1 copper-binding periplasmic metallochaperone CueP -
LJF68_RS21735 (4450025) 4450025..4450237 - 213 WP_000014594.1 RNA chaperone/antiterminator CspA -
LJF68_RS21740 (4450525) 4450525..4450815 - 291 WP_000455790.1 HTH-type transcriptional regulator -
LJF68_RS21745 (4451254) 4451254..4451964 + 711 WP_000190524.1 DUF3053 domain-containing protein -
LJF68_RS21750 (4452014) 4452014..4452988 - 975 WP_000804678.1 glyoxylate/hydroxypyruvate reductase GhrB -
LJF68_RS21755 (4453207) 4453207..4453869 - 663 WP_000747548.1 OmpA family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(31-152)

Antitoxin

Vibrio phage ICP1, Orf50

(5-94)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17731.46 Da        Isoelectric Point: 9.8719

>T221901 WP_000533912.1 NZ_CP085816:4448456-4448941 [Salmonella enterica subsp. enterica serovar Enteritidis]
VGRVTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEVTGNLRRNM
PDPIPVIILARLAVDLSFHGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKSSQTQQRTLFLRLP
Q

Download         Length: 486 bp

>T221901 NZ_CP117620:c2526445-2526343 [Escherichia albertii]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAAAACA
GGAATCGTTTTCGGTCTCTTTTT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10950.60 Da        Isoelectric Point: 4.6811

>AT221901 WP_000965886.1 NZ_CP085816:4448181-4448468 [Salmonella enterica subsp. enterica serovar Enteritidis]
MLYKGCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILETACQAAEKVILDRRVFNFNDEQYEEFINLLDAPVADD
PVIEKLLARKPQWDV

Download         Length: 288 bp

>AT221901 NZ_CP117620:2526341-2526573 [Escherichia albertii]
ATAAAAAGAGACCGAAAACGATTCCTGTTTTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAA
TAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCGTTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1R2W0C7

Antitoxin

Source ID Structure
PDB 7AK8
AlphaFold DB A0A3V2JDX2

References