Detailed information of TA system TA221895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3555140..3555716
• Replicon: chromosome
• Accession: NZ_CP085816
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Slowpoke

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: LJF68_RS17500
• Protein ID: WP_001131963.1
• Coordinates: 3555429..3555716 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: LJF68_RS17495
• Protein ID: WP_000063142.1
• Coordinates: 3555140..3555442 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF68_RS17480 (3551650)	3551650..3553800	+	2151	WP_000379928.1	pyruvate/proton symporter BtsT	-
LJF68_RS17485 (3553895)	3553895..3554098	+	204	WP_000460616.1	YbdD/YjiX family protein	-
LJF68_RS17490 (3554109)	3554109..3555065	+	957	WP_000187843.1	GTPase	-
LJF68_RS17495 (3555140)	3555140..3555442	-	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
LJF68_RS17500 (3555429)	3555429..3555716	-	288	WP_001131963.1	BrnT family toxin	Toxin
LJF68_RS17505 (3556133)	3556133..3557390	+	1258	Protein_3415	NERD domain-containing protein/DEAD/DEAH box helicase	-
LJF68_RS17510 (3557384)	3557384..3557563	+	180	Protein_3416	DNA methyltransferase	-
LJF68_RS17515 (3557553)	3557553..3558857	+	1305	WP_000863534.1	type I restriction-modification protein specificity subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T221895 WP_001131963.1 NZ_CP085816:c3555716-3555429 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T221895 NZ_CP117620:c1091239-1091021 [Escherichia albertii]
ATGTCCGATAAACCTTTAACAAAAACCGATTATTTGATGCGTCTGCGTCGTTGCCAGACAATTGACACGCTGGAACGTGT
TATCGAGAAAAATAAATATGAATTATCAGATAATGAATTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTTGCAGAGC
TGACTATGAATAAGCTATATGACAAGATCCCTTCCTCAGTGTGGAAATTCATTCGGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT221895 WP_000063142.1 NZ_CP085816:c3555442-3555140 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT221895 NZ_CP117620:c1091639-1091265 [Escherichia albertii]
ATGGATGAATACTCACCAAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGTAACCATGGCTGGGTAAATGACCCAACCTCGGCAATCAATCTACAGCTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAACAGATTGATGAATATCTG
GACGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAACCGATT
GTTCCGTTGCTTTGTTAACGCCACCAAAGCGAATCCTGCAAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01