22187

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3195130..3195817 Replicon chromosome
Accession NC_009525
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag MRA_RS15270 Protein ID WP_003414624.1
Coordinates 3195374..3195817 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag MRA_RS15265 Protein ID WP_003414620.1
Coordinates 3195130..3195387 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRA_RS15245 3190450..3191304 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
MRA_RS15250 3191360..3192523 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
MRA_RS15255 3192540..3193754 - 1215 WP_003899518.1 zinc metalloprotease Rip -
MRA_RS15260 3193762..3195003 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
MRA_RS15265 3195130..3195387 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
MRA_RS15270 3195374..3195817 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
MRA_RS15275 3195897..3196559 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
MRA_RS15280 3196655..3196846 + 192 WP_003414632.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MRA_RS15285 3197178..3198926 + 1749 WP_178121166.1 cytochrome c biogenesis protein DipZ -
MRA_RS15290 3199022..3199603 + 582 WP_003414644.1 fasciclin domain-containing protein -
MRA_RS15295 3199703..3199969 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T22187 WP_003414624.1 NC_009525:3195374-3195817 [Mycobacterium tuberculosis H37Ra]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T22187 NC_009525:3195374-3195817 [Mycobacterium tuberculosis H37Ra]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT22187 WP_003414620.1 NC_009525:3195130-3195387 [Mycobacterium tuberculosis H37Ra]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT22187 NC_009525:3195130-3195387 [Mycobacterium tuberculosis H37Ra]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
AlphaFold DB G0TFH0

References