Detailed information of TA system TA022186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3189529..3190077
• Replicon: chromosome
• Accession: NC_009525
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TFG5
• Locus tag: MRA_RS15240
• Protein ID: WP_003414602.1
• Coordinates: 3189814..3190077 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: O33347
• Locus tag: MRA_RS15235
• Protein ID: WP_003414599.1
• Coordinates: 3189529..3189810 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRA_RS15210	3185152..3186009	-	858	WP_003899513.1	type I methionyl aminopeptidase	-
MRA_RS15215	3186051..3186635	-	585	WP_003899514.1	DUF1707 domain-containing protein	-
MRA_RS15220	3186739..3186987	+	249	WP_003913411.1	antitoxin VapB23	-
MRA_RS15225	3186984..3187364	+	381	WP_003414592.1	type II toxin-antitoxin system VapC family toxin	-
MRA_RS15230	3187446..3189257	-	1812	WP_003414596.1	penicillin-binding protein	-
MRA_RS15235	3189529..3189810	+	282	WP_003414599.1	type II toxin-antitoxin system antitoxin RelF	Antitoxin
MRA_RS15240	3189814..3190077	+	264	WP_003414602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MRA_RS15245	3190450..3191304	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
MRA_RS15250	3191360..3192523	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
MRA_RS15255	3192540..3193754	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
MRA_RS15260	3193762..3195003	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10204.82 Da
• Isoelectric Point: 11.5078

>T22186 WP_003414602.1 NC_009525:3189814-3190077 [Mycobacterium tuberculosis H37Ra]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Nucleotide

• Length: 264 bp

>T22186 NC_009525:3189814-3190077 [Mycobacterium tuberculosis H37Ra]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGCATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10183.40 Da
• Isoelectric Point: 4.3440

>AT22186 WP_003414599.1 NC_009525:3189529-3189810 [Mycobacterium tuberculosis H37Ra]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Nucleotide

• Length: 282 bp

>AT22186 NC_009525:3189529-3189810 [Mycobacterium tuberculosis H37Ra]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Structures

Toxin

• PDB: 3G5O

Antitoxin

• AlphaFold DB: A0A7U4BWP8