Detailed information of TA system TA022185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3148612..3149216
• Replicon: chromosome
• Accession: NC_009525
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: MRA_RS15040
• Protein ID: WP_003414492.1
• Coordinates: 3148612..3149004 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P71622
• Locus tag: MRA_RS15045
• Protein ID: WP_003912003.1
• Coordinates: 3149001..3149216 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRA_RS15010	3143761..3144549	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
MRA_RS15015	3144883..3145428	-	546	WP_003913758.1	DUF1802 family protein	-
MRA_RS15020	3145701..3146585	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
MRA_RS15025	3146588..3147475	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
MRA_RS15030	3147780..3148325	-	546	WP_003899500.1	DUF1802 family protein	-
MRA_RS15035	3148322..3148591	-	270	WP_003414489.1	DUF2277 family protein	-
MRA_RS15040	3148612..3149004	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
MRA_RS15045	3149001..3149216	-	216	WP_003912003.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MRA_RS15050	3149263..3150012	+	750	WP_003414497.1	enoyl-CoA hydratase	-
MRA_RS15055	3150091..3151173	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
MRA_RS15060	3151166..3152476	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
MRA_RS15065	3152479..3153306	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
MRA_RS15070	3153303..3154214	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T22185 WP_003414492.1 NC_009525:c3149004-3148612 [Mycobacterium tuberculosis H37Ra]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T22185 NC_009525:c3149004-3148612 [Mycobacterium tuberculosis H37Ra]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7483.50 Da
• Isoelectric Point: 4.9732

>AT22185 WP_003912003.1 NC_009525:c3149216-3149001 [Mycobacterium tuberculosis H37Ra]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAADDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT22185 NC_009525:c3149216-3149001 [Mycobacterium tuberculosis H37Ra]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGCGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: P71622