Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3148612..3149216 Replicon chromosome
Accession NC_009525
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag MRA_RS15040 Protein ID WP_003414492.1
Coordinates 3148612..3149004 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P71622
Locus tag MRA_RS15045 Protein ID WP_003912003.1
Coordinates 3149001..3149216 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRA_RS15010 3143761..3144549 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
MRA_RS15015 3144883..3145428 - 546 WP_003913758.1 DUF1802 family protein -
MRA_RS15020 3145701..3146585 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
MRA_RS15025 3146588..3147475 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
MRA_RS15030 3147780..3148325 - 546 WP_003899500.1 DUF1802 family protein -
MRA_RS15035 3148322..3148591 - 270 WP_003414489.1 DUF2277 family protein -
MRA_RS15040 3148612..3149004 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
MRA_RS15045 3149001..3149216 - 216 WP_003912003.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MRA_RS15050 3149263..3150012 + 750 WP_003414497.1 enoyl-CoA hydratase -
MRA_RS15055 3150091..3151173 - 1083 WP_003414499.1 ABC transporter ATP-binding protein -
MRA_RS15060 3151166..3152476 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
MRA_RS15065 3152479..3153306 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
MRA_RS15070 3153303..3154214 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T22185 WP_003414492.1 NC_009525:c3149004-3148612 [Mycobacterium tuberculosis H37Ra]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T22185 NC_009525:c3149004-3148612 [Mycobacterium tuberculosis H37Ra]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7483.50 Da        Isoelectric Point: 4.9732

>AT22185 WP_003912003.1 NC_009525:c3149216-3149001 [Mycobacterium tuberculosis H37Ra]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAADDDELFTTGVSWNVS

Download         Length: 216 bp

>AT22185 NC_009525:c3149216-3149001 [Mycobacterium tuberculosis H37Ra]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGCGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB P71622

References