Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3122135..3122705 Replicon chromosome
Accession NC_009525
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag MRA_RS14890 Protein ID WP_003414166.1
Coordinates 3122135..3122491 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag MRA_RS14895 Protein ID WP_003901465.1
Coordinates 3122475..3122705 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRA_RS14870 3117587..3119275 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
MRA_RS14875 3119279..3119605 - 327 WP_003414157.1 hypothetical protein -
MRA_RS14880 3119778..3120365 + 588 WP_003914429.1 DUF3558 family protein -
MRA_RS14885 3120384..3122033 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
MRA_RS14890 3122135..3122491 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
MRA_RS14895 3122475..3122705 - 231 WP_003901465.1 antitoxin MazE Antitoxin
MRA_RS14900 3122748..3123791 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
MRA_RS22390 3123982..3124257 + 276 WP_003911993.1 DUF1778 domain-containing protein -
MRA_RS22795 3124433..3124687 - 255 WP_003917684.1 hypothetical protein -
MRA_RS14915 3124835..3125239 + 405 WP_009938577.1 hypothetical protein -
MRA_RS14920 3125236..3125427 + 192 WP_003414184.1 hypothetical protein -
MRA_RS14925 3125491..3126780 + 1290 Protein_2917 transposase family protein -
MRA_RS14930 3127014..3127271 + 258 WP_003899489.1 hypothetical protein -
MRA_RS14935 3127376..3127687 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T22183 WP_003414166.1 NC_009525:c3122491-3122135 [Mycobacterium tuberculosis H37Ra]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T22183 NC_009525:c3122491-3122135 [Mycobacterium tuberculosis H37Ra]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT22183 WP_003901465.1 NC_009525:c3122705-3122475 [Mycobacterium tuberculosis H37Ra]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT22183 NC_009525:c3122705-3122475 [Mycobacterium tuberculosis H37Ra]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References