Detailed information of TA system TA221819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3599313..3599863
• Replicon: chromosome
• Accession: NZ_CP085813
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Minun

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: M7RJ32
• Locus tag: LJF39_RS17715
• Protein ID: WP_001199743.1
• Coordinates: 3599313..3599621 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7RP97
• Locus tag: LJF39_RS17720
• Protein ID: WP_001118105.1
• Coordinates: 3599624..3599863 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF39_RS23055 (3594661)	3594661..3594936	-	276	WP_235784153.1	fimbria/pilus outer membrane usher protein	-
LJF39_RS17690 (3594869)	3594869..3595870	-	1002	WP_235784150.1	fimbria/pilus outer membrane usher protein	-
LJF39_RS17695 (3595887)	3595887..3596627	-	741	WP_001676217.1	SEF14/SEF18 fimbria chaperone SefB	-
LJF39_RS17700 (3596749)	3596749..3597279	-	531	WP_000909461.1	SEF14 fimbria major subunit SefA	-
LJF39_RS17705 (3597602)	3597602..3598735	+	1134	Protein_3456	IS3 family transposase	-
LJF39_RS17710 (3598767)	3598767..3598907	-	141	Protein_3457	Arm DNA-binding domain-containing protein	-
LJF39_RS17715 (3599313)	3599313..3599621	-	309	WP_001199743.1	CcdB family protein	Toxin
LJF39_RS17720 (3599624)	3599624..3599863	-	240	WP_001118105.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LJF39_RS17725 (3599973)	3599973..3600221	-	249	WP_000168388.1	ribbon-helix-helix domain-containing protein	-
LJF39_RS17730 (3600299)	3600299..3600844	-	546	WP_223151225.1	helix-turn-helix domain-containing protein	-
LJF39_RS17745 (3601612)	3601612..3602619	+	1008	Protein_3462	NAD(P)-dependent alcohol dehydrogenase	-
LJF39_RS17750 (3602589)	3602589..3603176	-	588	WP_227790970.1	gluconokinase	-
LJF39_RS17755 (3603393)	3603393..3604424	+	1032	WP_000453347.1	L-idonate 5-dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3597694..3600799	3105

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11845.67 Da
• Isoelectric Point: 6.7228

>T221819 WP_001199743.1 NZ_CP085813:c3599621-3599313 [Salmonella enterica subsp. enterica serovar Enteritidis]
MQYMVYRNKGNSKAYPYLLDVQSDIIDELHTRMVIPLFPVSRLVNNPVKRLTPTLNVEGNDYLVMTHEMASIRLSQIGDE
VMDVRSHRQTIKNALDFIFDGF

Nucleotide

• Length: 309 bp

>T221819 NZ_CP117600:c637850-637464 [Escherichia albertii]
ATGATTTGGGTCAGTGCACAGGAGGTCATTGCCTTCCACGATCGAATTTTGCAACGTTTTCCCGGTGTGGCGGGCCTGGC
CGACCCCGGCAGAGCACAGGCGCTGATTTATCGCGTGCAGAACCGTGTTCACTACGAGGGCGTTACTGACCTGTTTGAAC
TGGCAGCAATCTACTGGGTTGCCATCGCTCGCGGTCACATCTTTCATGATGGTAATAAACGTACCGCATTTTTCGTTACT
ATGACCTTTCTTTGGCGCAATGGCATCCGTATCCGTGACGTTGATAACTCGCTGGAGAACCTGACTGTCGAAGCGGCTAC
CGGAGAAAAAACGGTCGGTCAATTAGCGCGATGTTTACGTGAACGAGTGGATAGTTCCGGGAATTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8719.90 Da
• Isoelectric Point: 6.4633

>AT221819 WP_001118105.1 NZ_CP085813:c3599863-3599624 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPGMSVRHKKTVSVTLEPALLQQARDAGINLSAVLTAALKEEISATGVERWKAENRAGLQELNRITDEHGLLSDDYRTF

Nucleotide

• Length: 240 bp

>AT221819 NZ_CP117600:c638068-637847 [Escherichia albertii]
ATGCAAACTTACACTTCCACACAAGCCAGAGCCAATATTGCCACTGTACTGGATACCGCGTTGAATGGTGAGCCAGTTGA
AATCACCCGTCGCGACGGCAGCTCTGCCGTGCTCATTAGTAAAGCCGCATTCGAGGCGTATCAAAATGCGAAACTGGACG
CGGAATTTGATGCCATCATGCAACGTCATGAACACACAATTGAAGCGTTGACTGATCGATGA

Structures

Toxin

• AlphaFold DB: A0A6C6Z9V9

Antitoxin

• AlphaFold DB: A0A5H9SZK8