Detailed information of TA system TA022181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3082138..3082817
• Replicon: chromosome
• Accession: NC_009525
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: MRA_RS14670
• Protein ID: WP_003414059.1
• Coordinates: 3082138..3082554 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: MRA_RS14675
• Protein ID: WP_003414061.1
• Coordinates: 3082551..3082817 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRA_RS14650	3078190..3079092	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
MRA_RS14655	3079161..3079913	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
MRA_RS14660	3080157..3080432	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
MRA_RS14665	3080429..3082051	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
MRA_RS14670	3082138..3082554	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
MRA_RS14675	3082551..3082817	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MRA_RS14680	3082843..3083238	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
MRA_RS14685	3083235..3083504	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
MRA_RS14690	3083514..3084608	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
MRA_RS14695	3084605..3085024	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
MRA_RS14700	3085098..3085577	-	480	WP_003414073.1	dihydrofolate reductase	-
MRA_RS14705	3085648..3086448	-	801	WP_003911953.1	thymidylate synthase	-
MRA_RS14710	3086604..3087341	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T22181 WP_003414059.1 NC_009525:c3082554-3082138 [Mycobacterium tuberculosis H37Ra]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T22181 NC_009525:c3082554-3082138 [Mycobacterium tuberculosis H37Ra]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT22181 WP_003414061.1 NC_009525:c3082817-3082551 [Mycobacterium tuberculosis H37Ra]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT22181 NC_009525:c3082817-3082551 [Mycobacterium tuberculosis H37Ra]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64