Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2881695..2882332 Replicon chromosome
Accession NC_009525
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag MRA_RS13595 Protein ID WP_003413180.1
Coordinates 2881695..2882090 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag MRA_RS13600 Protein ID WP_003413183.1
Coordinates 2882087..2882332 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRA_RS13555 2877098..2878309 + 1212 WP_009936235.1 alpha/beta hydrolase family protein -
MRA_RS13560 2878436..2879095 + 660 WP_003900846.1 LppA family lipoprotein -
MRA_RS13565 2879092..2879754 + 663 WP_003900848.1 hypothetical protein -
MRA_RS13570 2879751..2880029 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
MRA_RS13575 2880122..2880535 + 414 WP_003413164.1 PIN domain nuclease -
MRA_RS13580 2880574..2880831 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
MRA_RS13585 2880828..2881205 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
MRA_RS13590 2881221..2881595 - 375 WP_003413177.1 hypothetical protein -
MRA_RS13595 2881695..2882090 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
MRA_RS13600 2882087..2882332 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
MRA_RS13605 2882743..2883162 - 420 WP_003413190.1 A24 family peptidase -
MRA_RS13610 2883174..2883983 - 810 WP_003413193.1 shikimate dehydrogenase -
MRA_RS13615 2883980..2885233 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
MRA_RS13620 2885226..2885738 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T22178 WP_003413180.1 NC_009525:c2882090-2881695 [Mycobacterium tuberculosis H37Ra]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T22178 NC_009525:c2882090-2881695 [Mycobacterium tuberculosis H37Ra]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT22178 WP_003413183.1 NC_009525:c2882332-2882087 [Mycobacterium tuberculosis H37Ra]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT22178 NC_009525:c2882332-2882087 [Mycobacterium tuberculosis H37Ra]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References