Detailed information of TA system TA022175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2866235..2866875
• Replicon: chromosome
• Accession: NC_009525
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: MRA_RS13490
• Protein ID: WP_003412970.1
• Coordinates: 2866235..2866654 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: MRA_RS13495
• Protein ID: WP_003412975.1
• Coordinates: 2866651..2866875 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRA_RS13465	2861820..2862542	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
MRA_RS13470	2863059..2863286	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
MRA_RS13475	2863283..2863684	+	402	WP_003412963.1	PIN domain-containing protein	-
MRA_RS13480	2863719..2864639	-	921	WP_003412965.1	restriction endonuclease	-
MRA_RS22765	2864980..2865225	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MRA_RS13485	2865284..2866234	+	951	WP_003911916.1	Lsr2 family protein	-
MRA_RS13490	2866235..2866654	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
MRA_RS13495	2866651..2866875	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
MRA_RS13500	2866906..2869749	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
MRA_RS13505	2869821..2870222	-	402	WP_003412981.1	hypothetical protein	-
MRA_RS13510	2870222..2870692	-	471	WP_003899364.1	transcription antitermination factor NusB	-
MRA_RS13515	2870695..2871258	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T22175 WP_003412970.1 NC_009525:c2866654-2866235 [Mycobacterium tuberculosis H37Ra]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T22175 NC_009525:c2866654-2866235 [Mycobacterium tuberculosis H37Ra]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT22175 WP_003412975.1 NC_009525:c2866875-2866651 [Mycobacterium tuberculosis H37Ra]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT22175 NC_009525:c2866875-2866651 [Mycobacterium tuberculosis H37Ra]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09