Detailed information of TA system TA221733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 45516..46132
• Replicon: chromosome
• Accession: NZ_CP085809
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain Raikou

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8Z2U3
• Locus tag: LJF43_RS00225
• Protein ID: WP_000238494.1
• Coordinates: 45758..46132 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8E9YHE9
• Locus tag: LJF43_RS00220
• Protein ID: WP_001523745.1
• Coordinates: 45516..45758 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF43_RS00185 (LJF43_00185)	40642..41241	+	600	WP_000965695.1	glucose-1-phosphatase	-
LJF43_RS00190 (LJF43_00190)	41235..42107	+	873	WP_000921423.1	virulence factor BrkB family protein	-
LJF43_RS00195 (LJF43_00195)	42104..42541	+	438	WP_000560975.1	D-aminoacyl-tRNA deacylase	-
LJF43_RS00200 (LJF43_00200)	42586..43527	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LJF43_RS00205 (LJF43_00205)	43542..43988	-	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	-
LJF43_RS00210 (LJF43_00210)	43985..44296	-	312	WP_000558160.1	type II toxin-antitoxin system HigB family toxin	-
LJF43_RS00215 (LJF43_00215)	44382..45311	-	930	WP_001162859.1	alpha/beta hydrolase	-
LJF43_RS00220 (LJF43_00220)	45516..45758	+	243	WP_001523745.1	CopG family transcriptional regulator	Antitoxin
LJF43_RS00225 (LJF43_00225)	45758..46132	+	375	WP_000238494.1	type II toxin-antitoxin system VapC family toxin	Toxin
LJF43_RS00230 (LJF43_00230)	46170..47099	-	930	WP_000027727.1	formate dehydrogenase accessory protein FdhE	-
LJF43_RS00235 (LJF43_00235)	47096..47731	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LJF43_RS00240 (LJF43_00240)	47728..48630	-	903	WP_000331359.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13908.18 Da
• Isoelectric Point: 7.3567

>T221733 WP_000238494.1 NZ_CP085809:45758-46132 [Salmonella enterica subsp. enterica serovar Typhimurium]
MVKGSALFDTNILIDLFSGRIEAKHALEAYPPQNAISLITWMEVMVGAKKYHQENRTRIALSAFNIIGVTQEIAERSVIV
RQEYGMKLPDAIILATAQVHRCELVTRNTKDFADIPGVITPYHL

Nucleotide

• Length: 375 bp

>T221733 NZ_CP117580:c271304-271002 [Escherichia albertii]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACACAAAAA
GATACCTTCTGATATTCATATTACATTGTTGCGGAAGCTGGATATTATCAATGCCGCAACTACCTGGAAGGACTTGAGAT
CACCACCAGGTAATCGGTATGAGGAATTGTCAGGGAAGCTAAACGGCTATTCATCAATAAGGGTGAATAATCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9437.77 Da
• Isoelectric Point: 4.4463

>AT221733 WP_001523745.1 NZ_CP085809:45516-45758 [Salmonella enterica subsp. enterica serovar Typhimurium]
MMAGMDMGRILLDLSDDVIKRLDDLKVQRNLPRAELLREAVEQYLERQDRAETTISKALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT221733 NZ_CP117580:c270930-270592 [Escherichia albertii]
ATGAAACAGGCAACCAGAAAACCGACGACTGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTTAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGCAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGACCTGTGG
GAAGTTGAAAATGATATGCGCGTTCAGGAAGAGTTAAGCCGTATCACTACTGCTGAAGAATTTATTTCTCAGCGGAATCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A5I6CND8