Detailed information of TA system TA022173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 2412095..2412624
• Replicon: chromosome
• Accession: NC_009525
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G0TMR4
• Locus tag: MRA_RS11355
• Protein ID: WP_003411124.1
• Coordinates: 2412095..2412412 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P9WJ74
• Locus tag: MRA_RS11360
• Protein ID: WP_003411127.1
• Coordinates: 2412409..2412624 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRA_RS11325	2407232..2408308	+	1077	WP_003411110.1	hypothetical protein	-
MRA_RS11330	2408305..2408586	+	282	WP_003411112.1	hypothetical protein	-
MRA_RS11335	2408622..2409695	+	1074	WP_003411116.1	quinone-dependent dihydroorotate dehydrogenase	-
MRA_RS11340	2409700..2410230	-	531	WP_003411119.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
MRA_RS11345	2410278..2411624	-	1347	WP_003411121.1	M20/M25/M40 family metallo-hydrolase	-
MRA_RS11355	2412095..2412412	-	318	WP_003411124.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MRA_RS11360	2412409..2412624	-	216	WP_003411127.1	antitoxin ParD2	Antitoxin
MRA_RS11365	2412879..2413937	+	1059	WP_003411129.1	hypothetical protein	-
MRA_RS11370	2414067..2414423	-	357	WP_003411130.1	hypothetical protein	-
MRA_RS11375	2414518..2415300	-	783	WP_003411131.1	cell wall synthesis protein Wag31	-
MRA_RS11380	2415568..2415858	-	291	WP_003900476.1	YggT family protein	-
MRA_RS11385	2416020..2416676	-	657	WP_003411133.1	cell division protein SepF	-
MRA_RS11390	2416742..2417518	-	777	WP_003411137.1	YggS family pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• Conserved hypothetical protein CHP02574, addiction module (11-69)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12312.88 Da
• Isoelectric Point: 6.4831

>T22173 WP_003411124.1 NC_009525:c2412412-2412095 [Mycobacterium tuberculosis H37Ra]
MTRRLRVHNGVEDDLFEAFSYYADAAPDQIDRLYNLFVDAVTKRIPQAPNAFAPLFKHYRHIYLRPFRYYVAYRTTDEAI
DILAVRHGMENPNAVEAEISGRTFE

Nucleotide

• Length: 318 bp

>T22173 NC_009525:c2412412-2412095 [Mycobacterium tuberculosis H37Ra]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7880.70 Da
• Isoelectric Point: 4.4055

>AT22173 WP_003411127.1 NC_009525:c2412624-2412409 [Mycobacterium tuberculosis H37Ra]
VVVNRALLASVDALSRDEQIELVEHINGNLAEGMHISEANQALIEARANDTDDAHWSTIDDFDKRIRARLG

Nucleotide

• Length: 216 bp

>AT22173 NC_009525:c2412624-2412409 [Mycobacterium tuberculosis H37Ra]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Toxin

• AlphaFold DB: G0TMR4

Antitoxin

• AlphaFold DB: A0A7U4FAJ4