Detailed information of TA system TA221729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4195306..4195922
• Replicon: chromosome
• Accession: NZ_CP085808
• Organism: Salmonella enterica subsp. enterica serovar Typhi strain Entei

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8Z2U3
• Locus tag: LJF44_RS20730
• Protein ID: WP_000238494.1
• Coordinates: 4195306..4195680 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8E9YHE9
• Locus tag: LJF44_RS20735
• Protein ID: WP_001523745.1
• Coordinates: 4195680..4195922 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF44_RS20715 (LJF44_20720)	4192808..4193710	+	903	WP_000331359.1	formate dehydrogenase subunit beta	-
LJF44_RS20720 (LJF44_20725)	4193707..4194342	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LJF44_RS20725 (LJF44_20730)	4194339..4195268	+	930	WP_000027727.1	formate dehydrogenase accessory protein FdhE	-
LJF44_RS20730 (LJF44_20735)	4195306..4195680	-	375	WP_000238494.1	type II toxin-antitoxin system VapC family toxin	Toxin
LJF44_RS20735 (LJF44_20740)	4195680..4195922	-	243	WP_001523745.1	CopG family transcriptional regulator	Antitoxin
LJF44_RS20740 (LJF44_20745)	4196127..4197056	+	930	WP_001162859.1	alpha/beta hydrolase	-
LJF44_RS20745 (LJF44_20750)	4197142..4197453	+	312	WP_000558160.1	type II toxin-antitoxin system HigB family toxin	-
LJF44_RS20750 (LJF44_20755)	4197450..4197896	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	-
LJF44_RS20755 (LJF44_20760)	4197911..4198852	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LJF44_RS20760 (LJF44_20765)	4198897..4199334	-	438	WP_000560975.1	D-aminoacyl-tRNA deacylase	-
LJF44_RS20765 (LJF44_20770)	4199331..4200203	-	873	WP_000921423.1	virulence factor BrkB family protein	-
LJF44_RS20770 (LJF44_20775)	4200197..4200796	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13908.18 Da
• Isoelectric Point: 7.3567

>T221729 WP_000238494.1 NZ_CP085808:c4195680-4195306 [Salmonella enterica subsp. enterica serovar Typhi]
MVKGSALFDTNILIDLFSGRIEAKHALEAYPPQNAISLITWMEVMVGAKKYHQENRTRIALSAFNIIGVTQEIAERSVIV
RQEYGMKLPDAIILATAQVHRCELVTRNTKDFADIPGVITPYHL

Nucleotide

• Length: 375 bp

>T221729 NZ_CP117576:c53560-53282 [Escherichia albertii]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCACATTCGTGAGTACATTGCGGAACAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCCGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCACATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9437.77 Da
• Isoelectric Point: 4.4463

>AT221729 WP_001523745.1 NZ_CP085808:c4195922-4195680 [Salmonella enterica subsp. enterica serovar Typhi]
MMAGMDMGRILLDLSDDVIKRLDDLKVQRNLPRAELLREAVEQYLERQDRAETTISKALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT221729 NZ_CP117576:c53829-53560 [Escherichia albertii]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGTCGTCAGCAACTCGAACGCGGCGATGTACTTCCCCATGATATAGTTGAATCCTCAGCGACTGCATGGCGGAAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A5I6CND8