Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2267945..2268571 Replicon chromosome
Accession NC_009525
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag MRA_RS10645 Protein ID WP_003410075.1
Coordinates 2268173..2268571 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag MRA_RS10640 Protein ID WP_003911750.1
Coordinates 2267945..2268172 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRA_RS10630 2265984..2266328 - 345 WP_003410065.1 ferredoxin family protein -
MRA_RS10635 2266517..2267686 - 1170 WP_003899126.1 ATP-binding protein -
MRA_RS10640 2267945..2268172 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MRA_RS10645 2268173..2268571 + 399 WP_003410075.1 PIN domain nuclease Toxin
MRA_RS10650 2268754..2269185 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
MRA_RS10655 2269286..2269720 + 435 WP_003914358.1 DUF1398 domain-containing protein -
MRA_RS22195 2270157..2270336 - 180 Protein_2090 hypothetical protein -
MRA_RS10660 2270397..2271044 + 648 WP_075631456.1 transposase -
MRA_RS10665 2270998..2271588 + 591 WP_003899131.1 IS110 family transposase -
MRA_RS10670 2271716..2272972 - 1257 WP_003410095.1 HNH endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T22169 WP_003410075.1 NC_009525:2268173-2268571 [Mycobacterium tuberculosis H37Ra]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T22169 NC_009525:2268173-2268571 [Mycobacterium tuberculosis H37Ra]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT22169 WP_003911750.1 NC_009525:2267945-2268172 [Mycobacterium tuberculosis H37Ra]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT22169 NC_009525:2267945-2268172 [Mycobacterium tuberculosis H37Ra]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References