Detailed information of TA system TA221660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3754216..3754836
• Replicon: chromosome
• Accession: NZ_CP085805
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Azumarill

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: LJF48_RS18505
• Protein ID: WP_001280991.1
• Coordinates: 3754618..3754836 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: V1H4V6
• Locus tag: LJF48_RS18500
• Protein ID: WP_000344807.1
• Coordinates: 3754216..3754590 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF48_RS18490 (3749355)	3749355..3750548	+	1194	WP_001039202.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
LJF48_RS18495 (3750571)	3750571..3753720	+	3150	WP_001132508.1	efflux RND transporter permease AcrB	-
LJF48_RS18500 (3754216)	3754216..3754590	+	375	WP_000344807.1	Hha toxicity modulator TomB	Antitoxin
LJF48_RS18505 (3754618)	3754618..3754836	+	219	WP_001280991.1	HHA domain-containing protein	Toxin
LJF48_RS18510 (3755015)	3755015..3755566	+	552	WP_001278793.1	maltose O-acetyltransferase	-
LJF48_RS18515 (3755684)	3755684..3756154	+	471	WP_000136183.1	YlaC family protein	-
LJF48_RS18520 (3756210)	3756210..3756350	-	141	WP_001197749.1	type B 50S ribosomal protein L36	-
LJF48_RS18525 (3756356)	3756356..3756616	-	261	WP_000801415.1	type B 50S ribosomal protein L31	-
LJF48_RS18530 (3756841)	3756841..3758391	+	1551	WP_000213140.1	EAL domain-containing protein	-
LJF48_RS18540 (3758622)	3758622..3759011	+	390	WP_000961287.1	MGMT family protein	-
LJF48_RS18545 (3759044)	3759044..3759613	-	570	WP_000779802.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T221660 WP_001280991.1 NZ_CP085805:3754618-3754836 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T221660 NZ_CP117562:c1794407-1793943 [Escherichia albertii]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAACCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACACTGCGCACCTACGGTAAAAAAACAGATGCCTATACAGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCAAGAGACAGAAGAAAAGCATTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14453.27 Da
• Isoelectric Point: 5.1444

>AT221660 WP_000344807.1 NZ_CP085805:3754216-3754590 [Salmonella enterica subsp. enterica serovar Enteritidis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT221660 NZ_CP117562:c1794742-1794407 [Escherichia albertii]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• PDB: 4ICG

Antitoxin

• AlphaFold DB: A0A5I5FGI0