Detailed information of TA system TA221645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 619306..619892
• Replicon: chromosome
• Accession: NZ_CP085805
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Azumarill

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A3V9X0E4
• Locus tag: LJF48_RS02885
• Protein ID: WP_000174964.1
• Coordinates: 619524..619892 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: V7IU48
• Locus tag: LJF48_RS02880
• Protein ID: WP_001522145.1
• Coordinates: 619306..619527 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF48_RS02855 (614327)	614327..615436	+	1110	WP_000822978.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LJF48_RS02860 (615496)	615496..616422	+	927	WP_000003007.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LJF48_RS02865 (616419)	616419..617696	+	1278	WP_000803764.1	branched chain amino acid ABC transporter permease LivM	-
LJF48_RS02870 (617693)	617693..618460	+	768	WP_000082080.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LJF48_RS02875 (618462)	618462..619175	+	714	WP_000416114.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LJF48_RS02880 (619306)	619306..619527	+	222	WP_001522145.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LJF48_RS02885 (619524)	619524..619892	+	369	WP_000174964.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LJF48_RS02890 (620151)	620151..621467	+	1317	WP_000624755.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LJF48_RS02895 (621572)	621572..622459	+	888	WP_000099303.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LJF48_RS02900 (622456)	622456..623301	+	846	WP_000572196.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LJF48_RS02905 (623303)	623303..624373	+	1071	WP_000907840.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	616419..625110	8691

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-114)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13601.91 Da
• Isoelectric Point: 6.7252

>T221645 WP_000174964.1 NZ_CP085805:619524-619892 [Salmonella enterica subsp. enterica serovar Enteritidis]
MTLQLISAEEIIQFHDRLLRVTPGVTGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLAANPDFVDMTVDAAAGRLTLEQIVVRLRA

Nucleotide

• Length: 369 bp

>T221645 NZ_CP117558:3497310-3497528 [Escherichia albertii]
ATGTCCGATAAACCTTTAACAAAAACCGATTATTTGATGCGTCTGCGTCGTTGCCAGACAATTGACACGCTGGAACGTGT
TATCGAGAAAAATAAATATGAATTATCAGATAATGAATTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTTGCAGAGC
TGACTATGAATAAGCTATATGACAAGATCCCTTCCTCAGTGTGGAAATTCATTCGGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8166.17 Da
• Isoelectric Point: 5.0775

>AT221645 WP_001522145.1 NZ_CP085805:619306-619527 [Salmonella enterica subsp. enterica serovar Enteritidis]
MRTVNYSEARQNLAEVLESAVTAGPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGNELRELADK

Nucleotide

• Length: 222 bp

>AT221645 NZ_CP117558:3496910-3497284 [Escherichia albertii]
ATGGATGAATACTCACCAAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGTAACCATGGCTGGGTAAATGACCCAACCTCGGCAATCAATCTACAGCTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAACAGATTGATGAATATCTG
GACGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAACCGATT
GTTCCGTTGCTTTGTTAACGCCACCAAAGCGAATCCTGCAAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A3V9X0E4