Detailed information of TA system TA022163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2204543..2205246
• Replicon: chromosome
• Accession: NC_009525
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: MRA_RS10260
• Protein ID: WP_003409778.1
• Coordinates: 2204543..2204872 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: MRA_RS21490
• Protein ID: WP_003409780.1
• Coordinates: 2204869..2205246 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRA_RS10240	2200926..2201996	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
MRA_RS10245	2201993..2202508	+	516	WP_003409718.1	flavin reductase family protein	-
MRA_RS10250	2202505..2203566	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
MRA_RS10255	2203563..2204333	+	771	WP_003409775.1	SDR family oxidoreductase	-
MRA_RS10260	2204543..2204872	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MRA_RS21490	2204869..2205246	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
MRA_RS10270	2205243..2205833	-	591	WP_003409784.1	SEC-C domain-containing protein	-
MRA_RS10275	2205888..2207252	+	1365	WP_003903691.1	HNH endonuclease	-
MRA_RS10280	2207407..2207859	-	453	WP_003899095.1	lipoprotein	-
MRA_RS10285	2207923..2208324	+	402	WP_003409869.1	hypothetical protein	-
MRA_RS10290	2208317..2208499	-	183	WP_003409870.1	hypothetical protein	-
MRA_RS10295	2208613..2208963	-	351	WP_003899096.1	hypothetical protein	-
MRA_RS10300	2208974..2209876	-	903	WP_003899097.1	hypothetical protein	-
MRA_RS10305	2209897..2210088	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T22163 WP_003409778.1 NC_009525:c2204872-2204543 [Mycobacterium tuberculosis H37Ra]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T22163 NC_009525:c2204872-2204543 [Mycobacterium tuberculosis H37Ra]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT22163 WP_003409780.1 NC_009525:c2205246-2204869 [Mycobacterium tuberculosis H37Ra]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT22163 NC_009525:c2205246-2204869 [Mycobacterium tuberculosis H37Ra]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8