Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 547390..547966 Replicon chromosome
Accession NZ_CP085804
Organism Salmonella enterica subsp. enterica serovar Enteritidis strain Leafeon

Toxin (Protein)


Gene name shpA Uniprot ID M7RG88
Locus tag LJF49_RS02580 Protein ID WP_001131963.1
Coordinates 547390..547677 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID B5R9R5
Locus tag LJF49_RS02585 Protein ID WP_000063142.1
Coordinates 547664..547966 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF49_RS02565 (544250) 544250..545554 - 1305 WP_000863534.1 type I restriction-modification protein specificity subunit -
LJF49_RS02570 (545544) 545544..545723 - 180 Protein_488 DNA methyltransferase -
LJF49_RS02575 (545717) 545717..546973 - 1257 Protein_489 NERD domain-containing protein/DEAD/DEAH box helicase -
LJF49_RS02580 (547390) 547390..547677 + 288 WP_001131963.1 BrnT family toxin Toxin
LJF49_RS02585 (547664) 547664..547966 + 303 WP_000063142.1 BrnA antitoxin family protein Antitoxin
LJF49_RS02590 (548041) 548041..548997 - 957 WP_000187843.1 GTPase -
LJF49_RS02595 (549008) 549008..549211 - 204 WP_000460616.1 YbdD/YjiX family protein -
LJF49_RS02600 (549306) 549306..551456 - 2151 WP_174580936.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11294.80 Da        Isoelectric Point: 9.5894

>T221621 WP_001131963.1 NZ_CP085804:547390-547677 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T221621 NZ_CP117556:c1953651-1953346 [Escherichia albertii]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCAGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TTCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11373.96 Da        Isoelectric Point: 10.1293

>AT221621 WP_000063142.1 NZ_CP085804:547664-547966 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp

>AT221621 NZ_CP117556:c1953871-1953653 [Escherichia albertii]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGCGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VD7

Antitoxin

Source ID Structure
PDB 7VD7
AlphaFold DB A0A4D6PB01

References