Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1766287..1766900 Replicon chromosome
Accession NC_009525
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag MRA_RS08290 Protein ID WP_003407786.1
Coordinates 1766496..1766900 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag MRA_RS08285 Protein ID WP_009935474.1
Coordinates 1766287..1766490 (+) Length 68 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRA_RS08255 1761692..1762072 + 381 WP_003916859.1 fumarate reductase subunit C -
MRA_RS08260 1762069..1762446 + 378 WP_003407771.1 fumarate reductase subunit FrdD -
MRA_RS08265 1762514..1763122 + 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
MRA_RS08270 1763306..1764454 + 1149 Protein_1615 MMPL family transporter -
MRA_RS08275 1764464..1764910 + 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
MRA_RS08280 1764945..1766234 + 1290 WP_003407781.1 threonine ammonia-lyase -
MRA_RS08285 1766287..1766490 + 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MRA_RS08290 1766496..1766900 + 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
MRA_RS08295 1766917..1768659 - 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
MRA_RS08300 1768652..1770949 - 2298 WP_003407790.1 malto-oligosyltrehalose synthase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-124)

Antitoxin

(2-44)


Sequences


Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T22160 WP_003407786.1 NC_009525:1766496-1766900 [Mycobacterium tuberculosis H37Ra]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T22160 NC_009525:1766496-1766900 [Mycobacterium tuberculosis H37Ra]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT22160 WP_009935474.1 NC_009525:1766287-1766490 [Mycobacterium tuberculosis H37Ra]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT22160 NC_009525:1766287-1766490 [Mycobacterium tuberculosis H37Ra]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6A7V


Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References