Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1687788..1688404 Replicon chromosome
Accession NC_009525
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag MRA_RS07945 Protein ID WP_003407593.1
Coordinates 1688087..1688404 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag MRA_RS07940 Protein ID WP_003900349.1
Coordinates 1687788..1688090 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRA_RS07930 1683674..1685521 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
MRA_RS07935 1685522..1687774 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
MRA_RS07940 1687788..1688090 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
MRA_RS07945 1688087..1688404 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
MRA_RS07950 1688401..1689405 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
MRA_RS07955 1689458..1690747 + 1290 WP_003407599.1 serine hydrolase -
MRA_RS07960 1690820..1691593 - 774 WP_003911559.1 class I SAM-dependent methyltransferase -
MRA_RS07965 1691651..1691863 - 213 WP_003898900.1 dodecin family protein -
MRA_RS22630 1691873..1692097 - 225 WP_160522526.1 NUDIX domain-containing protein -
MRA_RS07975 1692367..1693395 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T22159 WP_003407593.1 NC_009525:1688087-1688404 [Mycobacterium tuberculosis H37Ra]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T22159 NC_009525:1688087-1688404 [Mycobacterium tuberculosis H37Ra]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT22159 WP_003900349.1 NC_009525:1687788-1688090 [Mycobacterium tuberculosis H37Ra]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT22159 NC_009525:1687788-1688090 [Mycobacterium tuberculosis H37Ra]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References