Detailed information of TA system TA221588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4558095..4558681
• Replicon: chromosome
• Accession: NZ_CP085802
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Wailmer

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A3V9X0E4
• Locus tag: LJF51_RS22190
• Protein ID: WP_000174964.1
• Coordinates: 4558313..4558681 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: V7IU48
• Locus tag: LJF51_RS22185
• Protein ID: WP_001522145.1
• Coordinates: 4558095..4558316 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF51_RS22160 (4553116)	4553116..4554225	+	1110	WP_000822978.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LJF51_RS22165 (4554285)	4554285..4555211	+	927	WP_000003007.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LJF51_RS22170 (4555208)	4555208..4556485	+	1278	WP_000803764.1	branched chain amino acid ABC transporter permease LivM	-
LJF51_RS22175 (4556482)	4556482..4557249	+	768	WP_000082080.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LJF51_RS22180 (4557251)	4557251..4557964	+	714	WP_000416114.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LJF51_RS22185 (4558095)	4558095..4558316	+	222	WP_001522145.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LJF51_RS22190 (4558313)	4558313..4558681	+	369	WP_000174964.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LJF51_RS22195 (4558940)	4558940..4560256	+	1317	WP_000624755.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LJF51_RS22200 (4560361)	4560361..4561248	+	888	WP_000099303.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LJF51_RS22205 (4561245)	4561245..4562090	+	846	WP_000572196.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LJF51_RS22210 (4562092)	4562092..4563162	+	1071	WP_000907840.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4553116..4563899	10783

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-114)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13601.91 Da
• Isoelectric Point: 6.7252

>T221588 WP_000174964.1 NZ_CP085802:4558313-4558681 [Salmonella enterica subsp. enterica serovar Enteritidis]
MTLQLISAEEIIQFHDRLLRVTPGVTGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLAANPDFVDMTVDAAAGRLTLEQIVVRLRA

Nucleotide

• Length: 369 bp

>T221588 NZ_CP117511:2697725-2697874 [Enterococcus faecalis]
ATGCGTATGCACGTATTTCCGAAATTTAGAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACAATTCTTGG
GTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8166.17 Da
• Isoelectric Point: 5.0775

>AT221588 WP_001522145.1 NZ_CP085802:4558095-4558316 [Salmonella enterica subsp. enterica serovar Enteritidis]
MRTVNYSEARQNLAEVLESAVTAGPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGNELRELADK

Nucleotide

• Length: 222 bp

>AT221588 NZ_CP117511:c2697991-2697807 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCA
ATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A3V9X0E4