Detailed information of TA system TA221562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4558234..4558820
• Replicon: chromosome
• Accession: NZ_CP085801
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Wailord

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A3V9X0E4
• Locus tag: LJF52_RS22185
• Protein ID: WP_000174964.1
• Coordinates: 4558452..4558820 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: V7IU48
• Locus tag: LJF52_RS22180
• Protein ID: WP_001522145.1
• Coordinates: 4558234..4558455 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF52_RS22155 (4553255)	4553255..4554364	+	1110	WP_000822978.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LJF52_RS22160 (4554424)	4554424..4555350	+	927	WP_000003007.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LJF52_RS22165 (4555347)	4555347..4556624	+	1278	WP_000803764.1	branched chain amino acid ABC transporter permease LivM	-
LJF52_RS22170 (4556621)	4556621..4557388	+	768	WP_000082080.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LJF52_RS22175 (4557390)	4557390..4558103	+	714	WP_000416114.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LJF52_RS22180 (4558234)	4558234..4558455	+	222	WP_001522145.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LJF52_RS22185 (4558452)	4558452..4558820	+	369	WP_000174964.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LJF52_RS22190 (4559079)	4559079..4560395	+	1317	WP_000624755.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LJF52_RS22195 (4560500)	4560500..4561387	+	888	WP_000099303.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LJF52_RS22200 (4561384)	4561384..4562229	+	846	WP_000572196.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LJF52_RS22205 (4562231)	4562231..4563301	+	1071	WP_000907840.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4553255..4564038	10783

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-114)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13601.91 Da
• Isoelectric Point: 6.7252

>T221562 WP_000174964.1 NZ_CP085801:4558452-4558820 [Salmonella enterica subsp. enterica serovar Enteritidis]
MTLQLISAEEIIQFHDRLLRVTPGVTGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLAANPDFVDMTVDAAAGRLTLEQIVVRLRA

Nucleotide

• Length: 369 bp

>T221562 NZ_CP117484:32603-32908 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGGTCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8166.17 Da
• Isoelectric Point: 5.0775

>AT221562 WP_001522145.1 NZ_CP085801:4558234-4558455 [Salmonella enterica subsp. enterica serovar Enteritidis]
MRTVNYSEARQNLAEVLESAVTAGPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGNELRELADK

Nucleotide

• Length: 222 bp

>AT221562 NZ_CP117484:32383-32601 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A3V9X0E4