Detailed information of TA system TA221501

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2865829..2866379
• Replicon: chromosome
• Accession: NZ_CP085799
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Swellow

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: M7RJ32
• Locus tag: LJF54_RS14100
• Protein ID: WP_001199743.1
• Coordinates: 2865829..2866137 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7RP97
• Locus tag: LJF54_RS14105
• Protein ID: WP_001118105.1
• Coordinates: 2866140..2866379 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF54_RS14080 (2862403)	2862403..2863143	-	741	WP_001676217.1	SEF14/SEF18 fimbria chaperone SefB	-
LJF54_RS14085 (2863265)	2863265..2863795	-	531	WP_000909461.1	SEF14 fimbria major subunit SefA	-
LJF54_RS14090 (2864118)	2864118..2865251	+	1134	Protein_2755	IS3 family transposase	-
LJF54_RS14095 (2865283)	2865283..2865423	-	141	Protein_2756	Arm DNA-binding domain-containing protein	-
LJF54_RS14100 (2865829)	2865829..2866137	-	309	WP_001199743.1	CcdB family protein	Toxin
LJF54_RS14105 (2866140)	2866140..2866379	-	240	WP_001118105.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LJF54_RS14110 (2866488)	2866488..2866736	-	249	WP_000168388.1	ribbon-helix-helix domain-containing protein	-
LJF54_RS14115 (2866813)	2866813..2867358	-	546	WP_223151225.1	helix-turn-helix domain-containing protein	-
LJF54_RS14130 (2868115)	2868115..2869134	+	1020	WP_000152558.1	NAD(P)-dependent alcohol dehydrogenase	-
LJF54_RS14135 (2869162)	2869162..2869692	-	531	WP_000896758.1	gluconokinase	-
LJF54_RS14140 (2869909)	2869909..2870940	+	1032	WP_000453347.1	L-idonate 5-dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2864210..2867313	3103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11845.67 Da
• Isoelectric Point: 6.7228

>T221501 WP_001199743.1 NZ_CP085799:c2866137-2865829 [Salmonella enterica subsp. enterica serovar Enteritidis]
MQYMVYRNKGNSKAYPYLLDVQSDIIDELHTRMVIPLFPVSRLVNNPVKRLTPTLNVEGNDYLVMTHEMASIRLSQIGDE
VMDVRSHRQTIKNALDFIFDGF

Nucleotide

• Length: 309 bp

>T221501 NZ_CP117400:c4717328-4717017 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8719.90 Da
• Isoelectric Point: 6.4633

>AT221501 WP_001118105.1 NZ_CP085799:c2866379-2866140 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPGMSVRHKKTVSVTLEPALLQQARDAGINLSAVLTAALKEEISATGVERWKAENRAGLQELNRITDEHGLLSDDYRTF

Nucleotide

• Length: 240 bp

>AT221501 NZ_CP117400:c4717016-4716726 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A6C6Z9V9

Antitoxin

• AlphaFold DB: A0A5H9SZK8