Detailed information of TA system TA221500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2821566..2822142
• Replicon: chromosome
• Accession: NZ_CP085799
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Swellow

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: LJF54_RS13890
• Protein ID: WP_001131963.1
• Coordinates: 2821855..2822142 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: LJF54_RS13885
• Protein ID: WP_000063142.1
• Coordinates: 2821566..2821868 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF54_RS13870 (2818076)	2818076..2820226	+	2151	WP_000379928.1	pyruvate/proton symporter BtsT	-
LJF54_RS13875 (2820321)	2820321..2820524	+	204	WP_000460616.1	YbdD/YjiX family protein	-
LJF54_RS13880 (2820535)	2820535..2821491	+	957	WP_000187843.1	GTPase	-
LJF54_RS13885 (2821566)	2821566..2821868	-	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
LJF54_RS13890 (2821855)	2821855..2822142	-	288	WP_001131963.1	BrnT family toxin	Toxin
LJF54_RS13895 (2822559)	2822559..2823817	+	1259	Protein_2716	NERD domain-containing protein/DEAD/DEAH box helicase	-
LJF54_RS13900 (2823811)	2823811..2823990	+	180	Protein_2717	DNA methyltransferase	-
LJF54_RS13905 (2823980)	2823980..2825284	+	1305	WP_000863534.1	type I restriction-modification protein specificity subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T221500 WP_001131963.1 NZ_CP085799:c2822142-2821855 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T221500 NZ_CP117400:c4396202-4395711 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGGAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT221500 WP_000063142.1 NZ_CP085799:c2821868-2821566 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT221500 NZ_CP117400:c4396492-4396199 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGCGCTGCCGCAGAGGAAGCGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01