Detailed information of TA system TA022150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1015989..1016608
• Replicon: chromosome
• Accession: NC_009525
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MRA_RS04815
• Protein ID: WP_003404726.1
• Coordinates: 1016174..1016608 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: MRA_RS04810
• Protein ID: WP_003898641.1
• Coordinates: 1015989..1016168 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRA_RS04800	1011462..1013042	+	1581	WP_003909313.1	serine hydrolase	-
MRA_RS04805	1013039..1015432	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
MRA_RS22575	1015705..1015863	+	159	WP_003404720.1	hypothetical protein	-
MRA_RS04810	1015989..1016168	+	180	WP_003898641.1	antitoxin	Antitoxin
MRA_RS04815	1016174..1016608	+	435	WP_003404726.1	SRPBCC family protein	Toxin
MRA_RS04820	1016706..1017479	+	774	WP_003404735.1	VOC family protein	-
MRA_RS04825	1017544..1017993	+	450	WP_003404738.1	hypothetical protein	-
MRA_RS04830	1018128..1018358	-	231	WP_003898642.1	hypothetical protein	-
MRA_RS04835	1018525..1020033	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
MRA_RS04840	1020035..1021273	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T22150 WP_003404726.1 NC_009525:1016174-1016608 [Mycobacterium tuberculosis H37Ra]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T22150 NC_009525:1016174-1016608 [Mycobacterium tuberculosis H37Ra]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT22150 WP_003898641.1 NC_009525:1015989-1016168 [Mycobacterium tuberculosis H37Ra]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT22150 NC_009525:1015989-1016168 [Mycobacterium tuberculosis H37Ra]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5