Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 717720..718369 Replicon chromosome
Accession NC_009525
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag MRA_RS03280 Protein ID WP_003403236.1
Coordinates 717974..718369 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag MRA_RS03275 Protein ID WP_003403235.1
Coordinates 717720..717974 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRA_RS22550 712846..713541 + 696 WP_003900189.1 galactose-1-phosphate uridylyltransferase -
MRA_RS22555 713538..714029 + 492 WP_012054156.1 galactose-1-phosphate uridylyltransferase -
MRA_RS03255 714026..715117 + 1092 WP_003906403.1 galactokinase -
MRA_RS03265 715512..716576 + 1065 WP_003898532.1 hypothetical protein -
MRA_RS03270 716680..717627 + 948 WP_003403232.1 DUF732 domain-containing protein -
MRA_RS03275 717720..717974 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
MRA_RS03280 717974..718369 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
MRA_RS03285 718463..719203 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
MRA_RS03290 719335..719595 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MRA_RS03295 719592..719999 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
MRA_RS03300 720071..721222 - 1152 WP_003403248.1 FIST C-terminal domain-containing protein -
MRA_RS03305 721315..723042 - 1728 WP_003900979.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T22143 WP_003403236.1 NC_009525:717974-718369 [Mycobacterium tuberculosis H37Ra]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T22143 NC_009525:717974-718369 [Mycobacterium tuberculosis H37Ra]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT22143 WP_003403235.1 NC_009525:717720-717974 [Mycobacterium tuberculosis H37Ra]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT22143 NC_009525:717720-717974 [Mycobacterium tuberculosis H37Ra]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References