Detailed information of TA system TA221420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1738423..1739043
• Replicon: chromosome
• Accession: NZ_CP085796
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Froakie

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: LJF57_RS08160
• Protein ID: WP_001280991.1
• Coordinates: 1738423..1738641 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: V1H4V6
• Locus tag: LJF57_RS08165
• Protein ID: WP_000344807.1
• Coordinates: 1738669..1739043 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF57_RS08120 (1733646)	1733646..1734215	+	570	WP_000779802.1	YbaY family lipoprotein	-
LJF57_RS08125 (1734248)	1734248..1734637	-	390	WP_000961287.1	MGMT family protein	-
LJF57_RS08135 (1734868)	1734868..1736418	-	1551	WP_000213140.1	EAL domain-containing protein	-
LJF57_RS08140 (1736643)	1736643..1736903	+	261	WP_000801415.1	type B 50S ribosomal protein L31	-
LJF57_RS08145 (1736909)	1736909..1737049	+	141	WP_001197749.1	type B 50S ribosomal protein L36	-
LJF57_RS08150 (1737105)	1737105..1737575	-	471	WP_000136183.1	YlaC family protein	-
LJF57_RS08155 (1737693)	1737693..1738244	-	552	WP_001278793.1	maltose O-acetyltransferase	-
LJF57_RS08160 (1738423)	1738423..1738641	-	219	WP_001280991.1	HHA domain-containing protein	Toxin
LJF57_RS08165 (1738669)	1738669..1739043	-	375	WP_000344807.1	Hha toxicity modulator TomB	Antitoxin
LJF57_RS08170 (1739539)	1739539..1742688	-	3150	WP_001132508.1	efflux RND transporter permease AcrB	-
LJF57_RS08175 (1742711)	1742711..1743904	-	1194	WP_001039202.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T221420 WP_001280991.1 NZ_CP085796:c1738641-1738423 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T221420 NZ_CP117390:2369711-2369995 [Salmonella enterica subsp. enterica serovar Uganda]
ATGACTTATAAGCTGGCATTTAACGAATCTGCACTAAAAGAGTGGAAAAAGCTGGGACATACTATACAGGAACAGTTTAA
AAAAAAGTTACGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGCGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAGTGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14453.27 Da
• Isoelectric Point: 5.1444

>AT221420 WP_000344807.1 NZ_CP085796:c1739043-1738669 [Salmonella enterica subsp. enterica serovar Enteritidis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT221420 NZ_CP117390:2369473-2369721 [Salmonella enterica subsp. enterica serovar Uganda]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACTGAACTGAAACGTGACCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• PDB: 4ICG

Antitoxin

• AlphaFold DB: A0A5I5FGI0