Detailed information of TA system TA221416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 1163073..1163649
• Replicon: chromosome
• Accession: NZ_CP085796
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Froakie

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: LJF57_RS05510
• Protein ID: WP_001131963.1
• Coordinates: 1163073..1163360 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: LJF57_RS05515
• Protein ID: WP_000063142.1
• Coordinates: 1163347..1163649 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF57_RS05495 (1159933)	1159933..1161237	-	1305	WP_000863534.1	type I restriction-modification protein specificity subunit	-
LJF57_RS05500 (1161227)	1161227..1161406	-	180	Protein_1058	DNA methyltransferase	-
LJF57_RS05505 (1161400)	1161400..1162656	-	1257	Protein_1059	NERD domain-containing protein/DEAD/DEAH box helicase	-
LJF57_RS05510 (1163073)	1163073..1163360	+	288	WP_001131963.1	BrnT family toxin	Toxin
LJF57_RS05515 (1163347)	1163347..1163649	+	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
LJF57_RS05520 (1163724)	1163724..1164680	-	957	WP_000187843.1	GTPase	-
LJF57_RS05525 (1164691)	1164691..1164894	-	204	WP_000460616.1	YbdD/YjiX family protein	-
LJF57_RS05530 (1164989)	1164989..1167139	-	2151	WP_000379928.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T221416 WP_001131963.1 NZ_CP085796:1163073-1163360 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T221416 NZ_CP117390:849409-849807 [Salmonella enterica subsp. enterica serovar Uganda]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCGGGCGCCGG
AGCGTAATCTTGCCGGCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT221416 WP_000063142.1 NZ_CP085796:1163347-1163649 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT221416 NZ_CP117390:849182-849409 [Salmonella enterica subsp. enterica serovar Uganda]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGATGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01