Detailed information of TA system TA221415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1118838..1119388
• Replicon: chromosome
• Accession: NZ_CP085796
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Froakie

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: M7RJ32
• Locus tag: LJF57_RS05300
• Protein ID: WP_001199743.1
• Coordinates: 1119080..1119388 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7RP97
• Locus tag: LJF57_RS05295
• Protein ID: WP_001118105.1
• Coordinates: 1118838..1119077 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF57_RS05260 (1114277)	1114277..1115308	-	1032	WP_000453347.1	L-idonate 5-dehydrogenase	-
LJF57_RS05265 (1115525)	1115525..1116055	+	531	WP_000896758.1	gluconokinase	-
LJF57_RS05270 (1116083)	1116083..1117102	-	1020	WP_000152558.1	NAD(P)-dependent alcohol dehydrogenase	-
LJF57_RS05285 (1117859)	1117859..1118404	+	546	WP_223151225.1	helix-turn-helix domain-containing protein	-
LJF57_RS05290 (1118481)	1118481..1118729	+	249	WP_000168388.1	ribbon-helix-helix domain-containing protein	-
LJF57_RS05295 (1118838)	1118838..1119077	+	240	WP_001118105.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LJF57_RS05300 (1119080)	1119080..1119388	+	309	WP_001199743.1	CcdB family protein	Toxin
LJF57_RS05305 (1119794)	1119794..1119934	+	141	Protein_1019	Arm DNA-binding domain-containing protein	-
LJF57_RS05310 (1119966)	1119966..1121099	-	1134	Protein_1020	IS3 family transposase	-
LJF57_RS05315 (1121422)	1121422..1121952	+	531	WP_000909461.1	SEF14 fimbria major subunit SefA	-
LJF57_RS05320 (1122074)	1122074..1122814	+	741	WP_001676217.1	SEF14/SEF18 fimbria chaperone SefB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	1117904..1121007	3103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11845.67 Da
• Isoelectric Point: 6.7228

>T221415 WP_001199743.1 NZ_CP085796:1119080-1119388 [Salmonella enterica subsp. enterica serovar Enteritidis]
MQYMVYRNKGNSKAYPYLLDVQSDIIDELHTRMVIPLFPVSRLVNNPVKRLTPTLNVEGNDYLVMTHEMASIRLSQIGDE
VMDVRSHRQTIKNALDFIFDGF

Nucleotide

• Length: 309 bp

>T221415 NZ_CP117390:839701-840114 [Salmonella enterica subsp. enterica serovar Uganda]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTTCTGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8719.90 Da
• Isoelectric Point: 6.4633

>AT221415 WP_001118105.1 NZ_CP085796:1118838-1119077 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPGMSVRHKKTVSVTLEPALLQQARDAGINLSAVLTAALKEEISATGVERWKAENRAGLQELNRITDEHGLLSDDYRTF

Nucleotide

• Length: 240 bp

>AT221415 NZ_CP117390:839454-839720 [Salmonella enterica subsp. enterica serovar Uganda]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A6C6Z9V9

Antitoxin

• AlphaFold DB: A0A5H9SZK8