Detailed information of TA system TA221393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 1863295..1863871
• Replicon: chromosome
• Accession: NZ_CP085795
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Frogadier

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: LJF59_RS08875
• Protein ID: WP_001131963.1
• Coordinates: 1863295..1863582 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: LJF59_RS08880
• Protein ID: WP_000063142.1
• Coordinates: 1863569..1863871 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF59_RS08860 (1860155)	1860155..1861459	-	1305	WP_000863534.1	type I restriction-modification protein specificity subunit	-
LJF59_RS08865 (1861449)	1861449..1861628	-	180	Protein_1707	DNA methyltransferase	-
LJF59_RS08870 (1861622)	1861622..1862878	-	1257	Protein_1708	NERD domain-containing protein/DEAD/DEAH box helicase	-
LJF59_RS08875 (1863295)	1863295..1863582	+	288	WP_001131963.1	BrnT family toxin	Toxin
LJF59_RS08880 (1863569)	1863569..1863871	+	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
LJF59_RS08885 (1863946)	1863946..1864902	-	957	WP_000187843.1	GTPase	-
LJF59_RS08890 (1864913)	1864913..1865116	-	204	WP_000460616.1	YbdD/YjiX family protein	-
LJF59_RS08895 (1865211)	1865211..1867361	-	2151	WP_000379928.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T221393 WP_001131963.1 NZ_CP085795:1863295-1863582 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T221393 NZ_CP117386:c4203050-4202766 [Salmonella enterica subsp. enterica serovar Uganda]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT221393 WP_000063142.1 NZ_CP085795:1863569-1863871 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT221393 NZ_CP117386:c4203282-4203040 [Salmonella enterica subsp. enterica serovar Uganda]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01