Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1863295..1863871 Replicon chromosome
Accession NZ_CP085795
Organism Salmonella enterica subsp. enterica serovar Enteritidis strain Frogadier

Toxin (Protein)


Gene name shpA Uniprot ID M7RG88
Locus tag LJF59_RS08875 Protein ID WP_001131963.1
Coordinates 1863295..1863582 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID B5R9R5
Locus tag LJF59_RS08880 Protein ID WP_000063142.1
Coordinates 1863569..1863871 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF59_RS08860 (1860155) 1860155..1861459 - 1305 WP_000863534.1 type I restriction-modification protein specificity subunit -
LJF59_RS08865 (1861449) 1861449..1861628 - 180 Protein_1707 DNA methyltransferase -
LJF59_RS08870 (1861622) 1861622..1862878 - 1257 Protein_1708 NERD domain-containing protein/DEAD/DEAH box helicase -
LJF59_RS08875 (1863295) 1863295..1863582 + 288 WP_001131963.1 BrnT family toxin Toxin
LJF59_RS08880 (1863569) 1863569..1863871 + 303 WP_000063142.1 BrnA antitoxin family protein Antitoxin
LJF59_RS08885 (1863946) 1863946..1864902 - 957 WP_000187843.1 GTPase -
LJF59_RS08890 (1864913) 1864913..1865116 - 204 WP_000460616.1 YbdD/YjiX family protein -
LJF59_RS08895 (1865211) 1865211..1867361 - 2151 WP_000379928.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11294.80 Da        Isoelectric Point: 9.5894

>T221393 WP_001131963.1 NZ_CP085795:1863295-1863582 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T221393 NZ_CP117386:c4203050-4202766 [Salmonella enterica subsp. enterica serovar Uganda]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11373.96 Da        Isoelectric Point: 10.1293

>AT221393 WP_000063142.1 NZ_CP085795:1863569-1863871 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp

>AT221393 NZ_CP117386:c4203282-4203040 [Salmonella enterica subsp. enterica serovar Uganda]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VD7

Antitoxin

Source ID Structure
PDB 7VD7
AlphaFold DB A0A4D6PB01

References