Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 696149..696795 Replicon chromosome
Accession NC_009525
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag MRA_RS03120 Protein ID WP_003403122.1
Coordinates 696149..696541 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag MRA_RS03125 Protein ID WP_003403125.1
Coordinates 696538..696795 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRA_RS03105 691811..693337 + 1527 WP_003403112.1 virulence factor Mce family protein -
MRA_RS03110 693400..694542 + 1143 WP_003911253.1 virulence factor Mce family protein -
MRA_RS03115 694547..696097 + 1551 WP_003403119.1 MCE family protein -
MRA_RS03120 696149..696541 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
MRA_RS03125 696538..696795 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
MRA_RS03130 696978..698213 - 1236 WP_003403128.1 ATP-binding protein -
MRA_RS03135 698464..698877 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
MRA_RS03140 698874..699110 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
MRA_RS03145 699214..699720 - 507 WP_003900973.1 two component system sensor kinase HK1 -
MRA_RS03150 699834..700364 - 531 WP_003917320.1 two component system sensor kinase HK2 -
MRA_RS03155 700348..701109 - 762 WP_003403148.1 two-component system response regulator TcrA -
MRA_RS03160 701166..701477 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T22139 WP_003403122.1 NC_009525:c696541-696149 [Mycobacterium tuberculosis H37Ra]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T22139 NC_009525:c696541-696149 [Mycobacterium tuberculosis H37Ra]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT22139 WP_003403125.1 NC_009525:c696795-696538 [Mycobacterium tuberculosis H37Ra]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT22139 NC_009525:c696795-696538 [Mycobacterium tuberculosis H37Ra]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References