Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4367808..4368568 Replicon chromosome
Accession NZ_CP085793
Organism Salmonella enterica subsp. enterica serovar Enteritidis strain Poochyena

Toxin (Protein)


Gene name tacT Uniprot ID A0A1R2W0C7
Locus tag LJF61_RS21315 Protein ID WP_000533912.1
Coordinates 4367808..4368293 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID M7RHS4
Locus tag LJF61_RS21320 Protein ID WP_000965886.1
Coordinates 4368281..4368568 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF61_RS21285 (4362880) 4362880..4363542 + 663 WP_000747548.1 OmpA family lipoprotein -
LJF61_RS21290 (4363761) 4363761..4364735 + 975 WP_000804678.1 glyoxylate/hydroxypyruvate reductase GhrB -
LJF61_RS21295 (4364785) 4364785..4365495 - 711 WP_000190524.1 DUF3053 domain-containing protein -
LJF61_RS21300 (4365934) 4365934..4366224 + 291 WP_000455790.1 HTH-type transcriptional regulator -
LJF61_RS21305 (4366512) 4366512..4366724 + 213 WP_000014594.1 RNA chaperone/antiterminator CspA -
LJF61_RS21310 (4366898) 4366898..4367437 + 540 WP_000047147.1 copper-binding periplasmic metallochaperone CueP -
LJF61_RS21315 (4367808) 4367808..4368293 - 486 WP_000533912.1 GNAT family N-acetyltransferase Toxin
LJF61_RS21320 (4368281) 4368281..4368568 - 288 WP_000965886.1 DUF1778 domain-containing protein Antitoxin
LJF61_RS21325 (4368746) 4368746..4369213 - 468 WP_000702452.1 GNAT family N-acetyltransferase -
LJF61_RS21330 (4369385) 4369385..4369783 - 399 Protein_4154 IS3 family transposase -
LJF61_RS21335 (4370173) 4370173..4372242 - 2070 WP_001291741.1 glycine--tRNA ligase subunit beta -
LJF61_RS21340 (4372252) 4372252..4373163 - 912 WP_001168551.1 glycine--tRNA ligase subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(31-152)

Antitoxin

Vibrio phage ICP1, Orf50

(5-94)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17731.46 Da        Isoelectric Point: 9.8719

>T221355 WP_000533912.1 NZ_CP085793:c4368293-4367808 [Salmonella enterica subsp. enterica serovar Enteritidis]
VGRVTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEVTGNLRRNM
PDPIPVIILARLAVDLSFHGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKSSQTQQRTLFLRLP
Q

Download         Length: 486 bp

>T221355 NZ_CP117382:2019225-2019328 [Salmonella enterica subsp. enterica serovar Uganda]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10950.60 Da        Isoelectric Point: 4.6811

>AT221355 WP_000965886.1 NZ_CP085793:c4368568-4368281 [Salmonella enterica subsp. enterica serovar Enteritidis]
MLYKGCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILETACQAAEKVILDRRVFNFNDEQYEEFINLLDAPVADD
PVIEKLLARKPQWDV

Download         Length: 288 bp

>AT221355 NZ_CP117382:c2019330-2019187 [Salmonella enterica subsp. enterica serovar Uganda]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1R2W0C7

Antitoxin

Source ID Structure
PDB 7AK8
AlphaFold DB A0A3V2JDX2

References