Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2304200..2304776 Replicon chromosome
Accession NZ_CP085792
Organism Salmonella enterica subsp. enterica serovar Enteritidis strain Mightyena

Toxin (Protein)


Gene name shpA Uniprot ID M7RG88
Locus tag LJF62_RS10950 Protein ID WP_001131963.1
Coordinates 2304200..2304487 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID B5R9R5
Locus tag LJF62_RS10955 Protein ID WP_000063142.1
Coordinates 2304474..2304776 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJF62_RS10935 (2301064) 2301064..2302368 - 1305 WP_000863534.1 type I restriction-modification protein specificity subunit -
LJF62_RS10940 (2302358) 2302358..2302537 - 180 Protein_2116 DNA methyltransferase -
LJF62_RS10945 (2302531) 2302531..2303787 - 1257 Protein_2117 NERD domain-containing protein/DEAD/DEAH box helicase -
LJF62_RS10950 (2304200) 2304200..2304487 + 288 WP_001131963.1 BrnT family toxin Toxin
LJF62_RS10955 (2304474) 2304474..2304776 + 303 WP_000063142.1 BrnA antitoxin family protein Antitoxin
LJF62_RS10960 (2304851) 2304851..2305807 - 957 WP_000187843.1 GTPase -
LJF62_RS10965 (2305818) 2305818..2306021 - 204 WP_000460616.1 YbdD/YjiX family protein -
LJF62_RS10970 (2306116) 2306116..2308266 - 2151 WP_000379928.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11294.80 Da        Isoelectric Point: 9.5894

>T221315 WP_001131963.1 NZ_CP085792:2304200-2304487 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T221315 NZ_CP117375:41808-42089 [Salmonella enterica subsp. enterica serovar Dublin]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11373.96 Da        Isoelectric Point: 10.1293

>AT221315 WP_000063142.1 NZ_CP085792:2304474-2304776 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp

>AT221315 NZ_CP117375:41567-41818 [Salmonella enterica subsp. enterica serovar Dublin]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VD7

Antitoxin

Source ID Structure
PDB 7VD7
AlphaFold DB A0A4D6PB01

References