Detailed information of TA system TA221315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2304200..2304776
• Replicon: chromosome
• Accession: NZ_CP085792
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain Mightyena

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: LJF62_RS10950
• Protein ID: WP_001131963.1
• Coordinates: 2304200..2304487 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: LJF62_RS10955
• Protein ID: WP_000063142.1
• Coordinates: 2304474..2304776 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJF62_RS10935 (2301064)	2301064..2302368	-	1305	WP_000863534.1	type I restriction-modification protein specificity subunit	-
LJF62_RS10940 (2302358)	2302358..2302537	-	180	Protein_2116	DNA methyltransferase	-
LJF62_RS10945 (2302531)	2302531..2303787	-	1257	Protein_2117	NERD domain-containing protein/DEAD/DEAH box helicase	-
LJF62_RS10950 (2304200)	2304200..2304487	+	288	WP_001131963.1	BrnT family toxin	Toxin
LJF62_RS10955 (2304474)	2304474..2304776	+	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
LJF62_RS10960 (2304851)	2304851..2305807	-	957	WP_000187843.1	GTPase	-
LJF62_RS10965 (2305818)	2305818..2306021	-	204	WP_000460616.1	YbdD/YjiX family protein	-
LJF62_RS10970 (2306116)	2306116..2308266	-	2151	WP_000379928.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T221315 WP_001131963.1 NZ_CP085792:2304200-2304487 [Salmonella enterica subsp. enterica serovar Enteritidis]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T221315 NZ_CP117375:41808-42089 [Salmonella enterica subsp. enterica serovar Dublin]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT221315 WP_000063142.1 NZ_CP085792:2304474-2304776 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT221315 NZ_CP117375:41567-41818 [Salmonella enterica subsp. enterica serovar Dublin]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01