221303

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 4100..4688 Replicon plasmid pRCH52-1
Accession NZ_CP085789
Organism Acinetobacter baumannii strain RCH52

Toxin (Protein)


Gene name brnT Uniprot ID A0A3G6Z3M6
Locus tag NLHDIDDJ_RS19290 Protein ID WP_000438826.1
Coordinates 4100..4387 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID A0A335NKQ9
Locus tag NLHDIDDJ_RS19295 Protein ID WP_024434403.1
Coordinates 4374..4688 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLHDIDDJ_RS19270 (NLHDIDDJ_04000) 145..1074 + 930 WP_000845850.1 replication initiation protein RepM -
NLHDIDDJ_RS19275 (NLHDIDDJ_04001) 1141..1686 + 546 WP_000736776.1 plasmid replication DNA-binding protein -
NLHDIDDJ_RS19280 (NLHDIDDJ_04002) 1914..3101 - 1188 WP_004856455.1 tetracycline efflux MFS transporter Tet(39) -
NLHDIDDJ_RS19285 (NLHDIDDJ_04003) 3167..3808 + 642 WP_004787586.1 TetR/AcrR family transcriptional regulator C-terminal domain-containing protein -
NLHDIDDJ_RS19290 (NLHDIDDJ_04004) 4100..4387 + 288 WP_000438826.1 BrnT family toxin Toxin
NLHDIDDJ_RS19295 (NLHDIDDJ_04005) 4374..4688 + 315 WP_024434403.1 BrnA antitoxin family protein Antitoxin
NLHDIDDJ_RS19300 (NLHDIDDJ_04006) 4816..7227 + 2412 WP_111818397.1 TonB-dependent receptor ZnuD2 -
NLHDIDDJ_RS19305 (NLHDIDDJ_04007) 7532..7993 + 462 WP_000761345.1 DIP1984 family protein -
NLHDIDDJ_RS19310 (NLHDIDDJ_04008) 8342..8797 - 456 WP_000932329.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(39) - 1..11164 11164


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11303.72 Da        Isoelectric Point: 6.0889

>T221303 WP_000438826.1 NZ_CP085789:4100-4387 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T221303 NZ_CP117373:c2730447-2730344 [Salmonella enterica subsp. enterica serovar Dublin]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12222.86 Da        Isoelectric Point: 7.2026

>AT221303 WP_024434403.1 NZ_CP085789:4374-4688 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTIPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT221303 NZ_CP117373:2730342-2730487 [Salmonella enterica subsp. enterica serovar Dublin]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G6Z3M6

Antitoxin

Source ID Structure
AlphaFold DB A0A335NKQ9

References