Detailed information of TA system TA221300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 34052..34640
• Replicon: plasmid pANL
• Accession: NZ_CP085786
• Organism: Synechococcus elongatus PCC 6301

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q8KUW2
• Locus tag: PCC6301pg_RS13725
• Protein ID: WP_011055129.1
• Coordinates: 34052..34345 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: PCC6301pg_RS13730
• Protein ID: WP_231621407.1
• Coordinates: 34335..34640 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCC6301pg_RS13695 (PCC6301pg_B033)	29450..30829	-	1380	WP_011377393.1	DUF1254 domain-containing protein	-
PCC6301pg_RS13700	31628..32206	+	579	WP_041674944.1	hypothetical protein	-
PCC6301pg_RS13705 (PCC6301pg_B035)	32982..33269	+	288	WP_011055126.1	hypothetical protein	-
PCC6301pg_RS13710 (PCC6301pg_B036)	33276..33503	-	228	WP_040113190.1	hypothetical protein	-
PCC6301pg_RS13715 (PCC6301pg_B037)	33638..33907	-	270	WP_011055128.1	tyrosine-type recombinase/integrase	-
PCC6301pg_RS13720	33845..34003	+	159	WP_231621409.1	hypothetical protein	-
PCC6301pg_RS13725 (PCC6301pg_B038)	34052..34345	+	294	WP_011055129.1	BrnT family toxin	Toxin
PCC6301pg_RS13730 (PCC6301pg_B039)	34335..34640	+	306	WP_231621407.1	BrnA antitoxin family protein	Antitoxin
PCC6301pg_RS13735 (PCC6301pg_B040)	35155..35586	-	432	WP_011055132.1	DUF2442 domain-containing protein	-
PCC6301pg_RS13740 (PCC6301pg_B041)	35794..35901	+	108	WP_231621408.1	DUF4351 domain-containing protein	-
PCC6301pg_RS13745 (PCC6301pg_B042)	36010..36984	-	975	WP_011055134.1	NAD(P)-dependent alcohol dehydrogenase	-
PCC6301pg_RS13750 (PCC6301pg_B043)	37212..37937	-	726	WP_011055135.1	DJ-1/PfpI family protein	-
PCC6301pg_RS13755 (PCC6301pg_B044)	38105..38737	+	633	WP_237280180.1	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..46368	46368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (39-96)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11647.22 Da
• Isoelectric Point: 8.4122

>T221300 WP_011055129.1 NZ_CP085786:34052-34345 [Synechococcus elongatus PCC 6301]
MEQYEWDEQKRLSNLNKHGLDFRAAALVLANRYRFDITTERNQEQRIQAFAYVMQVLAVLTVVYRPGKIPRIISFRPASR
TEREIYYEWLETNPHDA

Nucleotide

• Length: 294 bp

>T221300 NZ_CP117373:824260-824673 [Salmonella enterica subsp. enterica serovar Dublin]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGTCAACACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11397.73 Da
• Isoelectric Point: 4.7238

>AT221300 WP_231621407.1 NZ_CP085786:34335-34640 [Synechococcus elongatus PCC 6301]
MTLEEMQNAIAARQDESDWEGVRQRIEAGLDPEPDEDSPDATELMRAALQQKRGRPPSLNPKTQIAIRLDRDIVEAFRAG
GPGWQTRMNAALREWLAAHPH

Nucleotide

• Length: 306 bp

>AT221300 NZ_CP117373:824013-824279 [Salmonella enterica subsp. enterica serovar Dublin]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: Q8KUW2