Detailed information of TA system TA022130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 4156524..4156916
• Replicon: chromosome
• Accession: NC_009523
• Organism: Roseiflexus sp. RS-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ROSERS_RS25195
• Protein ID: WP_011958011.1
• Coordinates: 4156524..4156670 (-)
• Length: 49 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A5UYG6
• Locus tag: ROSERS_RS17020
• Protein ID: WP_011958012.1
• Coordinates: 4156674..4156916 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ROSERS_RS17010	4152024..4154096	+	2073	WP_011958009.1	serine/threonine protein kinase	-
ROSERS_RS17015	4154184..4156202	+	2019	WP_041333974.1	hypothetical protein	-
ROSERS_RS25195	4156524..4156670	-	147	WP_011958011.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ROSERS_RS17020	4156674..4156916	-	243	WP_011958012.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ROSERS_RS17025	4157320..4158282	+	963	WP_011958013.1	IS110 family transposase	-
ROSERS_RS17030	4158451..4160178	-	1728	WP_011958014.1	alpha-glucosidase	-
ROSERS_RS17035	4160209..4160769	-	561	WP_011958015.1	HD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4157320..4158282	962

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-45)

Antitoxin

• SpoVT-AbrB domain (6-48)

Sequences

Toxin

Protein

• Length: 49 a.a.
• Molecular weight: 5302.01 Da
• Isoelectric Point: 6.4432

>T22130 WP_011958011.1 NC_009523:c4156670-4156524 [Roseiflexus sp. RS-1]
MSYVPDSGDIVWITFNPQAGHEQAGHRPALVLSPKSYNEKVGLALFYP

Nucleotide

• Length: 147 bp

>T22130 NC_009523:c4156670-4156524 [Roseiflexus sp. RS-1]
ATGTCATACGTTCCCGATAGCGGTGATATTGTGTGGATTACGTTCAACCCCCAGGCAGGGCATGAACAGGCGGGACATAG
ACCTGCTCTGGTCTTGTCGCCGAAGTCGTATAACGAAAAAGTTGGGCTGGCTTTATTTTATCCGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.14 Da
• Isoelectric Point: 4.1605

>AT22130 WP_011958012.1 NC_009523:c4156916-4156674 [Roseiflexus sp. RS-1]
MLTKVQKWGDSLALRIPATVAAAMRLEDNSSVEISLIKSQMIITPVSTTDWTLEELLAGITQENLYTEIETGRATGNEIW

Nucleotide

• Length: 243 bp

>AT22130 NC_009523:c4156916-4156674 [Roseiflexus sp. RS-1]
ATGCTCACCAAAGTTCAAAAATGGGGCGACAGTCTTGCTCTGCGAATACCAGCAACCGTCGCCGCCGCAATGCGTCTGGA
AGACAACTCGTCTGTTGAGATAAGCCTTATCAAAAGCCAGATGATCATTACGCCGGTTTCAACAACCGATTGGACACTGG
AGGAACTGCTGGCAGGGATCACCCAGGAGAATCTTTACACGGAAATCGAGACAGGTCGGGCGACAGGCAATGAAATCTGG
TGA

Structures

Antitoxin

• AlphaFold DB: A5UYG6