Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 31218..31855 Replicon plasmid p48411CZ_VIM_1
Accession NZ_CP085783
Organism Enterobacter hormaechei strain 48411CZ

Toxin (Protein)


Gene name vagD Uniprot ID A0A9E6WXZ4
Locus tag LJU35_RS23485 Protein ID WP_032662169.1
Coordinates 31218..31628 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A9E6WTF5
Locus tag LJU35_RS23490 Protein ID WP_032662167.1
Coordinates 31625..31855 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU35_RS23465 (LJU35_23465) 26584..26934 + 351 WP_032662182.1 DUF305 domain-containing protein -
LJU35_RS23470 (LJU35_23470) 27120..28028 - 909 WP_032662179.1 HNH endonuclease -
LJU35_RS23475 (LJU35_23475) 28575..29597 - 1023 WP_032662174.1 helicase UvrD -
LJU35_RS23480 (LJU35_23480) 29582..31144 - 1563 WP_032662171.1 AAA family ATPase -
LJU35_RS23485 (LJU35_23485) 31218..31628 - 411 WP_032662169.1 PIN domain-containing protein Toxin
LJU35_RS23490 (LJU35_23490) 31625..31855 - 231 WP_032662167.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LJU35_RS23495 (LJU35_23495) 32397..32687 + 291 WP_013087126.1 hypothetical protein -
LJU35_RS23500 (LJU35_23500) 32818..33348 + 531 WP_013087127.1 hypothetical protein -
LJU35_RS23505 (LJU35_23505) 33367..34143 + 777 WP_015572054.1 site-specific integrase -
LJU35_RS23510 (LJU35_23510) 34345..34602 + 258 Protein_36 site-specific integrase -
LJU35_RS23515 (LJU35_23515) 34846..35856 - 1011 WP_015572055.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / catA2 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B - 1..134366 134366


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-127)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14729.03 Da        Isoelectric Point: 5.7351

>T221298 WP_032662169.1 NZ_CP085783:c31628-31218 [Enterobacter hormaechei]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAEVSLASQEAGPAAQKLADAFAARLDAILPWDRTAV
DATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Download         Length: 411 bp

>T221298 NZ_CP117373:310110-310478 [Salmonella enterica subsp. enterica serovar Dublin]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGAGCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCACTATATCCCGTGGGTATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8645.77 Da        Isoelectric Point: 4.4692

>AT221298 WP_032662167.1 NZ_CP085783:c31855-31625 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAERGDVVSDEGRVNL

Download         Length: 231 bp

>AT221298 NZ_CP117373:309892-310113 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References