Detailed information of TA system TA221286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2350545..2351284
• Replicon: chromosome
• Accession: NZ_CP085780
• Organism: Enterobacter hormaechei strain 48411CZ

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A801DRD7
• Locus tag: LJU35_RS11335
• Protein ID: WP_017693417.1
• Coordinates: 2350545..2351030 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: LJU35_RS11340
• Protein ID: WP_003857131.1
• Coordinates: 2351018..2351284 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU35_RS11325 (LJU35_11325)	2346178..2346639	-	462	WP_045345185.1	response regulator	-
LJU35_RS11330 (LJU35_11330)	2346632..2349994	-	3363	WP_045345184.1	ATP-binding protein	-
LJU35_RS11335 (LJU35_11335)	2350545..2351030	-	486	WP_017693417.1	GNAT family N-acetyltransferase	Toxin
LJU35_RS11340 (LJU35_11340)	2351018..2351284	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
LJU35_RS11345 (LJU35_11345)	2351348..2352277	-	930	WP_017693416.1	LysR family transcriptional regulator	-
LJU35_RS11350 (LJU35_11350)	2352407..2353789	+	1383	WP_045345182.1	MFS transporter	-
LJU35_RS11355 (LJU35_11355)	2353811..2354806	-	996	WP_045345181.1	DUF2891 domain-containing protein	-
LJU35_RS11360 (LJU35_11360)	2354816..2355802	-	987	WP_045345180.1	DUF979 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2319159..2351284	32125

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17570.26 Da
• Isoelectric Point: 9.9658

>T221286 WP_017693417.1 NZ_CP085780:c2351030-2350545 [Enterobacter hormaechei]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKTFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T221286 NZ_CP117371:312767-313135 [Salmonella enterica subsp. enterica serovar Newport]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTATCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCACTATATCCCGTGGGTATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAACAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT221286 WP_003857131.1 NZ_CP085780:c2351284-2351018 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT221286 NZ_CP117371:312549-312770 [Salmonella enterica subsp. enterica serovar Newport]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTAACGGCGGGCCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGAAGCTGGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9