Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 1716044..1716841 Replicon chromosome
Accession NZ_CP085780
Organism Enterobacter hormaechei strain 48411CZ

Toxin (Protein)


Gene name KacT Uniprot ID A0A4Q2R3N2
Locus tag LJU35_RS08095 Protein ID WP_032670168.1
Coordinates 1716320..1716841 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A331QJY7
Locus tag LJU35_RS08090 Protein ID WP_015570876.1
Coordinates 1716044..1716313 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU35_RS08055 (LJU35_08055) 1711584..1711943 + 360 WP_017384779.1 helix-turn-helix domain-containing protein -
LJU35_RS08060 (LJU35_08060) 1712022..1712117 - 96 Protein_1560 transcriptional regulator -
LJU35_RS08065 (LJU35_08065) 1712270..1713511 + 1242 WP_039271154.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
LJU35_RS08070 (LJU35_08070) 1713544..1713771 - 228 WP_006809324.1 YccJ family protein -
LJU35_RS08075 (LJU35_08075) 1713790..1714386 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
LJU35_RS08080 (LJU35_08080) 1714778..1714948 + 171 WP_001273664.1 general stress protein -
LJU35_RS08085 (LJU35_08085) 1715065..1715970 + 906 WP_017693666.1 DMT family transporter -
LJU35_RS08090 (LJU35_08090) 1716044..1716313 + 270 WP_015570876.1 DUF1778 domain-containing protein Antitoxin
LJU35_RS08095 (LJU35_08095) 1716320..1716841 + 522 WP_032670168.1 GNAT family N-acetyltransferase Toxin
LJU35_RS08100 (LJU35_08100) 1716900..1718222 - 1323 WP_045344078.1 pyrimidine utilization transport protein G -
LJU35_RS08105 (LJU35_08105) 1718244..1718735 - 492 WP_015570873.1 pyrimidine utilization flavin reductase protein F -
LJU35_RS08110 (LJU35_08110) 1718748..1719338 - 591 WP_045344080.1 malonic semialdehyde reductase -
LJU35_RS08115 (LJU35_08115) 1719348..1720148 - 801 WP_045344082.1 pyrimidine utilization protein D -
LJU35_RS08120 (LJU35_08120) 1720156..1720542 - 387 WP_015570870.1 pyrimidine utilization protein C -
LJU35_RS08125 (LJU35_08125) 1720554..1721243 - 690 WP_045344084.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19437.18 Da        Isoelectric Point: 7.4318

>T221285 WP_032670168.1 NZ_CP085780:1716320-1716841 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLVRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T221285 NZ_CP117370:4803699-4804010 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10247.75 Da        Isoelectric Point: 5.8836

>AT221285 WP_015570876.1 NZ_CP085780:1716044-1716313 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWIAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT221285 NZ_CP117370:4804007-4804453 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q2R3N2

Antitoxin

Source ID Structure
AlphaFold DB A0A331QJY7

References