Detailed information of TA system TA221283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 631840..632416
• Replicon: chromosome
• Accession: NZ_CP085780
• Organism: Enterobacter hormaechei strain 48411CZ

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A800YKM1
• Locus tag: LJU35_RS03030
• Protein ID: WP_015572580.1
• Coordinates: 631840..632127 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A801DSF4
• Locus tag: LJU35_RS03035
• Protein ID: WP_017694570.1
• Coordinates: 632114..632416 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU35_RS03005 (LJU35_03005)	626928..627647	+	720	WP_017694573.1	winged helix-turn-helix domain-containing protein	-
LJU35_RS03010 (LJU35_03010)	627797..628267	+	471	WP_015572576.1	MarR family transcriptional regulator	-
LJU35_RS03015 (LJU35_03015)	628264..629331	+	1068	WP_026094404.1	HlyD family secretion protein	-
LJU35_RS03020 (LJU35_03020)	629321..630397	+	1077	WP_015572578.1	DUF2955 domain-containing protein	-
LJU35_RS03025 (LJU35_03025)	630394..631665	-	1272	WP_045344431.1	DUF445 domain-containing protein	-
LJU35_RS03030 (LJU35_03030)	631840..632127	+	288	WP_015572580.1	BrnT family toxin	Toxin
LJU35_RS03035 (LJU35_03035)	632114..632416	+	303	WP_017694570.1	BrnA antitoxin family protein	Antitoxin
LJU35_RS03040 (LJU35_03040)	632445..633083	-	639	WP_017694569.1	LysE family translocator	-
LJU35_RS03045 (LJU35_03045)	633122..633874	-	753	WP_015572582.1	AraC family transcriptional regulator	-
LJU35_RS03050 (LJU35_03050)	634028..635398	+	1371	WP_017694568.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
LJU35_RS03055 (LJU35_03055)	635580..636125	-	546	WP_006810254.1	YfaZ family outer membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11296.81 Da
• Isoelectric Point: 7.3587

>T221283 WP_015572580.1 NZ_CP085780:631840-632127 [Enterobacter hormaechei]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIRIIS
ARKADRKERRRYEHR

Nucleotide

• Length: 288 bp

>T221283 NZ_CP117370:c4441329-4440838 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGGAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11482.13 Da
• Isoelectric Point: 10.4094

>AT221283 WP_017694570.1 NZ_CP085780:632114-632416 [Enterobacter hormaechei]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT221283 NZ_CP117370:c4441619-4441326 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGCGCTGCCGCAGAGGAAGCGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA