Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 108010..109633 Replicon plasmid p48947CZ_IncHI2
Accession NZ_CP085778
Organism Enterobacter hormaechei strain 48947CZ

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag LJU36_RS24155 Protein ID WP_000088044.1
Coordinates 108308..109633 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag LJU36_RS24150 Protein ID WP_012477377.1
Coordinates 108010..108303 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU36_RS24130 (LJU36_24130) 103809..104201 + 393 WP_000341066.1 hypothetical protein -
LJU36_RS24135 (LJU36_24135) 104860..105516 + 657 WP_000462754.1 trypsin-like serine protease -
LJU36_RS24140 (LJU36_24140) 105719..106216 + 498 WP_000340139.1 membrane protein -
LJU36_RS24145 (LJU36_24145) 106221..107609 + 1389 WP_000083579.1 hypothetical protein -
LJU36_RS24150 (LJU36_24150) 108010..108303 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
LJU36_RS24155 (LJU36_24155) 108308..109633 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
LJU36_RS24160 (LJU36_24160) 109694..109900 + 207 WP_000134171.1 hypothetical protein -
LJU36_RS24165 (LJU36_24165) 110001..110411 + 411 WP_000985909.1 hypothetical protein -
LJU36_RS24170 (LJU36_24170) 110424..111239 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
LJU36_RS24175 (LJU36_24175) 111492..111917 + 426 WP_000278471.1 hypothetical protein -
LJU36_RS24180 (LJU36_24180) 112466..112774 + 309 WP_001224686.1 hypothetical protein -
LJU36_RS24185 (LJU36_24185) 112790..113647 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 / ant(3'')-Ia / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / qnrB1 / dfrA14 / tet(A) - 1..343120 343120


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T221282 WP_000088044.1 NZ_CP085778:108308-109633 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T221282 NZ_CP117370:c4231047-4230763 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT221282 WP_012477377.1 NZ_CP085778:108010-108303 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT221282 NZ_CP117370:c4231279-4231037 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References