Detailed information of TA system TA221268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 445809..446425
• Replicon: chromosome
• Accession: NZ_CP085774
• Organism: Enterobacter hormaechei strain 48947CZ

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LJU36_RS02135
• Protein ID: WP_015569913.1
• Coordinates: 445809..446180 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: LJU36_RS02140
• Protein ID: WP_015569912.1
• Coordinates: 446183..446425 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU36_RS02120 (LJU36_02120)	443309..444211	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
LJU36_RS02125 (LJU36_02125)	444208..444843	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
LJU36_RS02130 (LJU36_02130)	444840..445769	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
LJU36_RS02135 (LJU36_02135)	445809..446180	-	372	WP_015569913.1	PIN domain-containing protein	Toxin
LJU36_RS02140 (LJU36_02140)	446183..446425	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
LJU36_RS02145 (LJU36_02145)	446624..447544	+	921	WP_015569911.1	alpha/beta hydrolase	-
LJU36_RS02150 (LJU36_02150)	447553..448494	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
LJU36_RS02155 (LJU36_02155)	448539..448976	-	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
LJU36_RS02160 (LJU36_02160)	448973..449854	-	882	WP_003861949.1	virulence factor BrkB family protein	-
LJU36_RS02165 (LJU36_02165)	449848..450447	-	600	WP_003861947.1	glucose-1-phosphatase	-
LJU36_RS02170 (LJU36_02170)	450566..451366	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13767.90 Da
• Isoelectric Point: 6.4882

>T221268 WP_015569913.1 NZ_CP085774:c446180-445809 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVSARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T221268 NZ_CP117369:c12228-11800 [Salmonella enterica subsp. enterica serovar Dublin]
ATGAAACAGCCACGTACGAAGACCTATATGCTCGATACCTGTATCTGTTCGTTCATCATGCGCGAGCAGTCGGAAGCGGT
GCTGAAGCGCCTTGAGCAGGCGGTGCTGCGCGGCCACCGTATCGTGATCTCGGCCATCACCTACTCCGAGATGCGCTTCG
GCGCCACCGGACCTAAGGCCTCGCCGCGTCTCGTCCAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCC
TGGGATCGCGCTGCGGTAGACGCCACCACGGAGATTAAGGTGGCACTGTGCCTCGCCGGGACGCCGATCGGTCCGAACGA
CACGGCGATTGCCGGGCATGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGG
GCCTGGTACTGGAAGACTGGGTAAGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT221268 WP_015569912.1 NZ_CP085774:c446425-446183 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT221268 NZ_CP117369:c12455-12225 [Salmonella enterica subsp. enterica serovar Dublin]
ATGAGAACCGTATCCATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAAATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCTGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGCTTTGAGCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3