Detailed information of TA system TA221256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4256095..4256834
• Replicon: chromosome
• Accession: NZ_CP085767
• Organism: Enterobacter hormaechei strain 49589CZ

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: LJU37_RS20410
• Protein ID: WP_045329830.1
• Coordinates: 4256095..4256580 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: LJU37_RS20415
• Protein ID: WP_047050951.1
• Coordinates: 4256568..4256834 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU37_RS20390 (LJU37_20390)	4252248..4253006	-	759	WP_047050948.1	trans-aconitate 2-methyltransferase	-
LJU37_RS20395 (LJU37_20395)	4253091..4253675	-	585	WP_047050949.1	NUDIX domain-containing protein	-
LJU37_RS20400 (LJU37_20400)	4253762..4254520	+	759	WP_015570520.1	DeoR/GlpR family DNA-binding transcription regulator	-
LJU37_RS20405 (LJU37_20405)	4254625..4255917	+	1293	WP_047050950.1	glycosyl hydrolase family 28 protein	-
LJU37_RS20410 (LJU37_20410)	4256095..4256580	-	486	WP_045329830.1	GNAT family N-acetyltransferase	Toxin
LJU37_RS20415 (LJU37_20415)	4256568..4256834	-	267	WP_047050951.1	DUF1778 domain-containing protein	Antitoxin
LJU37_RS20420 (LJU37_20420)	4256898..4257827	-	930	WP_047050986.1	LysR family transcriptional regulator	-
LJU37_RS20425 (LJU37_20425)	4257957..4259345	+	1389	WP_047050952.1	MFS transporter	-
LJU37_RS20430 (LJU37_20430)	4259367..4260362	-	996	WP_047050954.1	DUF2891 domain-containing protein	-
LJU37_RS20435 (LJU37_20435)	4260372..4261358	-	987	WP_227788419.1	DUF979 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17512.18 Da
• Isoelectric Point: 9.9658

>T221256 WP_045329830.1 NZ_CP085767:c4256580-4256095 [Enterobacter hormaechei]
VGRVTAPEPLSSAHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T221256 NZ_CP117367:c1024902-1024375 [Salmonella enterica subsp. enterica serovar Dublin]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTACAACGCCATGCACTACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGCGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10089.51 Da
• Isoelectric Point: 4.4857

>AT221256 WP_047050951.1 NZ_CP085767:c4256834-4256568 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVANDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT221256 NZ_CP117367:c1025189-1024899 [Salmonella enterica subsp. enterica serovar Dublin]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGACTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA