Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3643877..3644674 Replicon chromosome
Accession NZ_CP085767
Organism Enterobacter hormaechei strain 49589CZ

Toxin (Protein)


Gene name KacT Uniprot ID A0A4Q2R3N2
Locus tag LJU37_RS17235 Protein ID WP_032670168.1
Coordinates 3644153..3644674 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5RT92
Locus tag LJU37_RS17230 Protein ID WP_001303459.1
Coordinates 3643877..3644146 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU37_RS17195 (LJU37_17195) 3639417..3639776 + 360 WP_040117469.1 helix-turn-helix domain-containing protein -
LJU37_RS17200 (LJU37_17200) 3639848..3639949 - 102 Protein_3330 transcriptional regulator -
LJU37_RS17205 (LJU37_17205) 3640102..3641343 + 1242 WP_047051685.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
LJU37_RS17210 (LJU37_17210) 3641376..3641603 - 228 WP_006809324.1 YccJ family protein -
LJU37_RS17215 (LJU37_17215) 3641622..3642218 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
LJU37_RS17220 (LJU37_17220) 3642610..3642780 + 171 WP_001273664.1 general stress protein -
LJU37_RS17225 (LJU37_17225) 3642897..3643802 + 906 WP_047051684.1 DMT family transporter -
LJU37_RS17230 (LJU37_17230) 3643877..3644146 + 270 WP_001303459.1 DUF1778 domain-containing protein Antitoxin
LJU37_RS17235 (LJU37_17235) 3644153..3644674 + 522 WP_032670168.1 GNAT family N-acetyltransferase Toxin
LJU37_RS17240 (LJU37_17240) 3644733..3646055 - 1323 WP_048982825.1 pyrimidine utilization transport protein G -
LJU37_RS17245 (LJU37_17245) 3646077..3646568 - 492 WP_047052688.1 pyrimidine utilization flavin reductase protein F -
LJU37_RS17250 (LJU37_17250) 3646581..3647171 - 591 WP_047052687.1 malonic semialdehyde reductase -
LJU37_RS17255 (LJU37_17255) 3647181..3647981 - 801 WP_047052686.1 pyrimidine utilization protein D -
LJU37_RS17260 (LJU37_17260) 3647989..3648375 - 387 WP_032670161.1 pyrimidine utilization protein C -
LJU37_RS17265 (LJU37_17265) 3648387..3649076 - 690 WP_047052685.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19437.18 Da        Isoelectric Point: 7.4318

>T221255 WP_032670168.1 NZ_CP085767:3644153-3644674 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLVRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T221255 NZ_CP117367:872683-873081 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10248.70 Da        Isoelectric Point: 5.8836

>AT221255 WP_001303459.1 NZ_CP085767:3643877-3644146 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT221255 NZ_CP117367:872456-872683 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q2R3N2

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7BCZ2

References